HPLC-ELSD同时测定不同产地醉鱼草果实中3种三萜皂苷的含量

HPLC-ELSD for determining contents of three triterpenoidal saponins in fruits of Buddleja lindleyana from different habitats

建立了HPLC-ELSD测定醉鱼草果实中三萜类成分含量的方法。采集不同产地醉鱼草果实药材共9批,采用反相高效液相色谱-蒸发光散射检测法测定其三萜成分。色谱条件：Waters Sun FireTMC18柱（4.6 mm×150 mm,5μm）,流动相为甲醇-水（82∶18）溶液,体积流量1 m L·min-1,柱温30℃。ELSD参数：漂移管温度106℃,喷雾温度50℃,载气（N2）流速1.5 L·min-1,增益系数1。结果表明醉鱼草果实中的3种三萜成分clinoposaponinⅢ,desrhamnoverbascosaponin,mimengosideⅠ的进样量分别在0.702~28.08μg（r=0.999 2）,0.390~15.60μg（r=0.998 9）,0.192~7.68μg（r=0.999 0）呈良好线性关系;平均加样回收率（n=6）分别为99.41%,99.08%,98.67%,相对标准偏差分别为0.86%,1.6%,1.8%。该方法简便、准确、可靠,适用于测定醉鱼草果实中三萜类成分的含量。

To establish an HPLC-ELSD method for the quantification of triterpenoids in the fruits of Buddleja lindleyana. The RPHPLC-ELSD method was used for the determination of triterpenoids in B. lindleyana fruits,which were collected from different habitats. The column used was a packed with 5 μm stationary phase Waters Sun FireTMC18（ 4. 6 mm × 150 mm,5 μm）. The mobile phase consisted of Methanol-water（ 82∶ 18） at a flow rate of 1 m L·min- 1. Column temperature： 30 ℃. ELSD conditions： drift tube temperature： 106 ℃; carrier gas（ nitrogen） flow rate： 1. 5 L·min- 1; amplification factor： 1. The calibration curves showed good linear relationship on a range from 0. 702 to 28. 08 μg（ r = 0. 999 2） for Clinoposaponin III,0. 390 to 15. 60 μg（ r = 0. 998 9） for Desrhamnoverbascosaponin and 0. 192 to 7. 68μg（ r = 0. 999 0） for Mimengoside I. The average recovery rate（ n = 6） were 99. 41%,99. 08%and 98. 67% and it＇s RSD were 0. 86%,1. 56% and 1. 80%. This method can be used to determine the contents of triterpenoids in the fruits of Buddleja lindleyana for its simplicity,accurateness and reliability.