玄参化学成分的HPLC-ESI-IT-TOF-MS分析

Identification of chemical constituents in Scrophulariae Radix by HPLC-IT-TOF-MS

应用HPLC-ESI-IT-TOF-MS技术对玄参中化学成分进行定性分析。采用Kromasil 100-5 C18色谱柱(4.6 mm×250mm,5μm),以乙腈-0.1%甲酸溶液为流动相梯度洗脱;质谱使用ESI离子源,正离子与负离子模式下采集数据,通过分析质谱信息及元素组成,并参考相关文献数据,共指认了64个化合物;其中新化合物2个,4-hydroxy-6-O-methylcatalpol和acetylangoroside C;玄参中首次报道的化合物17个,其中环烯醚萜苷类12个,苯丙素苷类3个,其他类2个。HPLC-ESI-IT-TOF-MS技术能快速准确地分析玄参中所含的主要成分,研究结果为阐明玄参的药效物质基础提供了依据。

This experiment was performed to establish a qualitative analysis on chemical constituents of Scrophulariae Radix by HPLC-ESI-IT-TOF-MS. The analysis was conducted on a C18column（ Kromasil 100-5,4. 6 mm × 250 mm,5 μm） with 0. 1% formic acid-acetonitrile as the mobile phase for gradient elution; ESI ion source was used for mass spectra,and data were collected innegative and positive modes. The results showed that 64 compounds from Scrophulariae Radix had been identified by analyzing negative ion mass data including element composition and by comparing with data from literature. Two new compounds（ 4-hydroxy-6-O-methylcatalpol and acetylangoroside C） and seventeen known compounds were detected from Scrophulariae Radix for the first time. Seventeen known compounds included twelve iridoid glycosides,three phenylpropanoid glycosides and two other kind compounds. This study will provide chemical basis for elucidation of the effective substance in the Scrophulariae Radix.