儿童脑胶质瘤组织中P27表达与病理分级、患儿预后的相关性研究

Relationship between P27 expression with tumor pathological grade and prognosis in pediatric brain gliomas

目的 探讨P2 7在儿童脑胶质瘤中的表达及与其发生、发展和患儿预后的关系。方法 应用免疫组织化学S -P法 ,检测 48例儿童脑胶质瘤和 9例正常脑组织中P2 7蛋白的表达 ,并结合长期随访资料进行分析。结果 P2 7在儿童脑胶质瘤阳性表达 (58.3 % )明显低于正常组 (1 0 0 % ) (P <0 .0 5) ;P2 7表达与儿童脑胶质瘤病理分级呈负相关 (r=0 .51 5 ,P <0 .0 1 ) ,随着肿瘤恶性程度的增高 ,P2 7表达呈降低态势 ;P2 7在低度恶性组与高度恶性组表达间有显著差异 (P <0 .0 5) ;P2 7的表达与脑胶质瘤的预后呈正相关 ,P2 7高表达组 ,患儿生存率高 ,反之则下降。结论 P2 7表达水平的降低在儿童脑胶质瘤发生、发展起重要作用 ,可作为判断儿童脑胶质瘤恶性程度及预测预后的参考指标。

Objectives To explore the expression of P27 in pediatric brain gliomas, as well as their possible roles in pathogenesis of gliomas and prognosis value.Methods The expression of P27 was investigated by immunohistochemical techniques in 9 cases of normal brain tissue and 48 cases of pediateic brain gliomas specimens fomalin-fixed and paraffin wax-embedded after surgy and patients' prognosis was evaluated by retrospective analysis combined with follow-up data.Results Positive expression percentage of P27 protein in pediatric brain gliomas was 58.3% and significantly lower than that in normal brain tissue(100% ,P<0.05).The expression of P27 protein was negatively correlated with pathological grade of gliomas (r=0.515,P<0.01).The survival rates of patients with high expression of P27 protein were enhanced.Conclusions P27 protein plays important roles in development and progression of pediatric brain gliomas and its expression level may be acted as a reference index for judging malignant degree and prognostic value of pediatric brain gliomas.