阿萨希毛孢子菌抗氧化酶活性研究

Study on antioxidant enzymatic activities of Trichosporon asahii

目的观察和测定44株不同来源阿萨希毛孢子菌(Trichosporon asahii,T.asahii)抗氧化酶活性。方法收集44株不同来源T.asahii,将其分为环境组(3株)、临床组(9株)、体内传代组(20株)和体外耐药组(12株),分别用羟胺法和可见光法测定其抗氧化酶超氧化物歧化酶(superoxide dismutase,SOD)和过氧化氢酶(catalase,CAT)活性,并进行比较和分析。结果环境组:SOD酶活性均值为(4.01±0.66)U/mgprot,CAT酶活性均值为(48.51±7.60)U/mgprot;临床组:SOD酶活性均值为(10.45±3.87)U/mgprot,CAT酶活性均值为(110.56±35.77)U/mgprot;体内传代组:至传代第5代,菌株SOD酶活性均值为(8.65±4.15)U/mgprot,CAT酶活性均值为(71.36±12.19)U/mgprot;耐药组:至诱导末期第10代菌株SOD酶活性均值为(8.02±1.56)U/mgprot,CAT酶活性均值为(80.43±8.92)U/mgprot。结论 T.asahii具有较强的抗氧化能力。不同来源T.asahii抗氧化能力不同,主要体现在抗氧化酶活性的不同。其中,临床组菌株抗氧化能力最强,体内传代组菌株和体外耐药组菌株次之,环境组菌株最低。

Objective To observe and determinate the antioxidant enzymatic activity of 44 Trichosporon asahii（T.asahii） strains from different sources. Methods Total 44 T.asahii strains collected from different sources were divided into four groups： environment group（3 strains）, clinical group（9 strains）, internal passage group（20 strains）, fl uconazole-resistant group（12 strains）, the superoxide dismutase（SOD） and catalase（CAT） activities were compared and analyzed by hydroxylamine method and visible light method, respectively. Results Environment group： SOD activity mean value was（4.01±0.66）U/mgprot, CAT activity mean value was（48.51±7.60）U/mgprot. Clinical group： SOD activity mean value was（10.45±3.87）U/mgprot, CAT activity mean value was（110.56±35.77）U/mgprot. Internal passage group： after the fi fth passage, SOD activity mean value was（8.65±4.15） U/mgprot, CAT activity mean value was（71.36±12.19）U/mgprot. Fluconazole-resistant group： after the tenth resistant induce, SOD activity mean value was（8.02±1.56）U/mgprot, CAT activity mean value was（80.43±8.92）U/mgprot. Conclusion T.asahii has strong antioxidant ability. T.asahii strains from different sources have different antioxidant abilities, which mainly manifested in the difference of antioxidant enzymatic activity. Clinical group strains has the strongest antioxidant capacity, internal passage group strains and fl uconazole-resistant group are the second, and the environmental group strains is the lowest.