白桦锌指蛋白基因的分离及表达分析

Identification and Expression Analysis of Genes Encoding Zinc Finger Proteins in Betula platyphylla

植物锌指蛋白是成员众多的转录因子家族,在植物的生长发育和对非生物胁迫响应等过程中具有重要作用。根据Cys和His残基的数目和位置将锌指转录因子分为C_2H_2、C_8、C6、C_3HC_4、C_2HC、C_2HC5、C_4、CCCH和C_4HC_3九种类型。本实验利用其他物种中已报导的植物逆境胁迫相关的锌指蛋白转录因子基因序列作为信息探针,在白桦基因组数据库中筛选出10个基因,分别是4个C_2H_2型、5个C_3HC_4-RING型和一个CCCH型。通过q RT-PCR技术对筛选的基因在根中的表达模式进行了分析,结果显示Bp ZFP_2、Bp ZFP_3、Bp ZFP_4、Bp ZFP5和Bp ZFP_8的表达受盐和干旱胁迫的显著诱导,表明这些基因参与了逆境胁迫的应答。

Zinc finger proteins constitute a large family of transcription factors and numerous members of them play diverse roles in many biological processes,including plant growth,development and stress responses.Based on their structural and functional diversities they were classified into nine types including C_2H_2,C_8,C_6,C_3HC_4,C_2 HC,C_2HC_5,C_4,CCCH and C_4HC_3. We studied the genes in Betula platyphylla database by the sequences and homology comparison of genes encoding zinc finger proteins involving in stress responses from other organisms. A total of ten genes（ designated as Bp ZFP1 to Bp ZFP_（10））,of which four were C_2H_2 type,five were C_3HC_4-RING type,one was CCCH type were identified. By quantitative RT-PCR assay,we analyzed the expression patterns of Bp ZFPs in roots. Bp ZFP_2,Bp ZFP_3,Bp ZFP_4,Bp ZFP_5 and Bp ZFP_8 were induced under high-salt and drought treatment significantly indicating their involvement in stress response in Birch.