TaCBL基因在小麦与叶锈菌互作过程中的表达分析

Expression analysis of TaCBL gene in defense response of wheat to Puccinia triticina

利用实时定量PCR技术对小麦与叶锈菌互作过程中的钙调磷酸酶B类似蛋白相关基因的表达进行了检测分析。结果表明,在亲和组合接种后的16h,TaCBL1、TaCBL3、TaCBL4、TaCBL7基因的相对表达量明显增加,分别达到了对照的近8,2,4.5,14倍的水平,而在不亲和组合中,这4个基因在不同时间点的表达水平与对照相近;TaCBL2和TaCBL9的表达趋势在亲和组合和不亲和组合间大致相似,但在不亲和组合中其表达的峰值出现的时间要早于亲和组合;TaCBL6在2个组合的表达变化基本一致。以上结果初步表明,TaCBL可能参与了小麦抵抗叶锈菌侵染的基础防卫反应,其中TaCBL2和TaCBL9在抵抗叶锈菌侵染过程中发挥正调控作用,而TaCBL1、TaCBL3、TaCBL4和TaCBL7的高表达削弱了寄主的抗病性从而更利于叶锈菌的侵染。为进一步深入研究Ca2+信号通路中钙调磷酸酶B类基因在小麦与叶锈菌互作过程中的作用机制奠定了试验基础。

In the interaction process of wheat and Puccinia triticina,RT-qPCR was used to detect gene expression of TaCBLin the experiment.The results indicated that 16 hafter the inoculation the relative expressions of TaCBL1,TaCBL3,TaCBL4 and TaCBL7 had a significant increase in the compatible combinations,respectively 8,2,4.5,14 times of the level of control.While in the incompatible combinations,the relative expressions of the above four genes had no significant difference with that of the control.In the two kinds of combinations,the expression trends of TaCBL2 and TaCBL9 were similar,but the peak time of TaCBL2 and TaCBL9in the incompatible combination was earlier than in the compatible combinations.The relative expression of TaCBL6 in the two combinations was basically identical.The results suggested TaCBL may be involved in the process of signal transduction during the interaction of wheat and Puccinia triticina on the basis of defense response.TaCBL2 and TaCBL9 may play apositive role in regulating the process of resistance to Puccinia triticinainfection,while the high expression of TaCBL1,TaCBL3,TaCBL4 and TaCBL7 weakened the resistance of the host,and became more conduc ive to leaf rust infection.All the results above laid a foundation for further study on the molecular mechanisms of Ca2＋in the interaction between the wheat and Pucciniatriticina.