高效液相色谱法同时测定人参健脾丸中人参皂苷Rg_1、Re、Rb_1和酸枣仁皂苷A、B的含量

Simultaneous Determination of Ginsenoside Rg_1,Re,Rb_1 and Jujuboside A,B in Renshen Jianpi Pellets by HPLC

目的建立高效液相色谱法同时测定人参健脾丸中人参皂苷Rg1、Re、Rb。和酸枣仁皂苷A、B5种有效成分的含量。方法采用Eclipse XDBC18（4．6mm×250mm，5μm）色谱柱，柱温35℃，以乙腈-水为流动相梯度洗脱，分段变体积流量测定，检测波长203nm。结果人参皂苷Rgl、Re、Rb1和酸枣仁皂苷A、B分别在0．32～2．24μg（r=0．9982）、0．16～1．12μg（r=0．9953）、0．32～2．24μg（r=0．9996）、0．16～1．12μg（r=0．9915）、0．08～0．56μg（r=0．9996）内与色谱峰面积呈良好的线性关系。平均回收率分别为97．18％、97．62％、98．79％、98．48％和94．51％，RSD分别为1．10％、0．98％、0．34％、1．09％和1．88％。结论首次建立的梯度洗脱同时检测人参皂苷Rg1、Re、Rb，和酸枣仁皂苷A、B含量方法准确、可靠，重复性好。可用于人参健脾丸的质量控制。

OBJECTIVE To establish an HPLC method for simultaneous determination of ginsenoside Rgl, Re, Rbl and jujubo- side A, B in Renshen Jianpi Pellets （RSJPW）. METHODS The determination was conducted on an Eclipse XDB C lscolumn （4. 6 mm × 250 ram, 5 μm） with the mobile phase of acetonitrile （A）-water （B） gradiendy eluted at the following flow rates ：0 -90 min, 1.0 mL · min-1 ; 90- 130 rain, 1.0 -0. 5 mL · min-1 ; 130 -140 min, 0. 5 mL · min-1. And the column temperature was main- tained at 35 ℃ ; the detection wavelength was set at 203 nm. RESULTS The calibration curves of ginsenoside Rgl, Re, Rbl and ju- juboside A, B were in good linearity over 0. 32 - 2.24 μg （ r = 0. 998 2）, 0. 16 - 1.12 μg （r = 0. 995 3）, 0. 32 -2. 24 μg （r = 0. 999 6）, 0. 16 - 1.12 μg （r =0. 991 5）, 0. 08 -0. 56 μg （r =0. 999 6）. The corresponding average recovery rates were 97.18%, 97, 62%, 98. 79% , 98. 48% , 94. 51% ; the standard deviations were 1.10% , 0.98%, 0.34% , 1.09% , 1.88% , respectively. CONCLUSION The established HPLC method is accurate, reliable and reproducible, which can be used as a reference for the qual- ity control of RSJPW.