溶酶体相关膜蛋白2抗体在抗中性粒细胞胞质抗体相关性小血管炎患者血清中的表达及临床意义

The level and clinical significance of serum anti-lysosomal associated membrane protein-2 antibody in patients with anti-neutrophil cytoplasmic antibodies-associated vasculitis

目的检测溶酶体相关膜蛋白2（LAMP-2）抗体在ANCA相关性小血管炎（AASV）患者血清中的表达并探讨其临床意义。方法选取20例AASV患者血清作为试验组，20名同期性别、年龄相匹配的健康体检者血清作为对照组。采用ELISA检测所有研究对象血清中LAMP-2抗体水平，对LAMP-2抗体水平与AASV患者ESR、CRP、伯明翰血管炎活动积分（BVAS）、血红蛋白、白蛋白等指标进行相关分析。2组间LAMP．2抗体水平比较采用t检验，LAPM-2抗体水平和ESR、CRP相关性检验采用Spearman相关分析，LAMP-2抗体水平和BVAS积分、血红蛋白、白蛋白相关性检验采用Pearson相关分析。结果①AASV患者血清中LAMP-2抗体水平[（3714±1446）pg／m1]高于对照组[（174±43）pg／m1]（t=10．94，P〈0．05），血红蛋白[（99±30）g／L]、白蛋白[（27±5）只，L]明显低于对照组[（138±14）g／L，（44±3）g／L]（t=5．27，t=13．04，P均〈0．05）。②AASV患者血清中LAMP-2抗体水平与BVAS积分呈正相关（r=0．6699，P〈0．05），与ESR、ClIP、血红蛋白、白蛋白无相关性（P〉0．05）。结论①LAMP-2抗体可能参与了AASV的发病过程；②LAMP-2抗体能反映AAsv疾病活动性，可作为血管炎活动的血清学临床监测指标。

Objective To explore the clinical significance of serum anti-lysosomal associated membrane protein-2 （LAMP-2） antibody in the pathogenesis of anti-neutrophil cytoplasmic antibodies （ANCA）- associated vasculitis （AASV） by investigating the relationship of its levels and AASV. Methods Sera from twenty patients with AASV and twenty healthy controls were collected. Serum anti-LAMP-2 antibody was detected using commercial enzyme linked immunosorbent assay （ELISA） kits. Anti-LAMP-2 antibody levels between the two groups were assessed using the t test, the correlation between anti-LAMP-2 antibody levels and erythrocyte sedimentation rate （ESR）, C-reactive protein （CRP） was assessed by Spearman＇s rank cor- relation test, the correlation between anti-LAMP-2 antibody levels and the Birmingham vasculitis activity score （BVAS）, hemoglobin （Hb）, albumin （Alb） was assessed by Pearson＇s rank correlation test. Results （1） The serum level of anti-LAMP-2 antibody in patients with AASV [（3 714±1 446） pg/ml] was higher than that in the healthy controls [（174±43） pg/ml] （t=10.94, P〈0.05）. The serum level of Hb [（99±30） g/L] and Alb [（27±5） g/L] in patients with AASV was lower than that in healthy controls [（138±14） g/L, （44±3） g/L] （t=5.27, t=13.04, P〉0.05）. （1） The level of anti-LAMP-2 antibody in AAS-V：：was positively correlated with BVAS （r=0.669 9, P〈0.05）, and was not found elevated compared with ESR, CRP, Hb and Alb （P〉0.05）. Conclusion （1） Anti-LAMP-2 antibody is involved in the pathogenesis of AASV. （2） Anti-LAMP-2 antibody is correlated with the activity of AASV, it may be an indicator of AASV disease activity.