摘要
目的检测溶酶体相关膜蛋白2(LAMP-2)抗体在ANCA相关性小血管炎(AASV)患者血清中的表达并探讨其临床意义。方法选取20例AASV患者血清作为试验组,20名同期性别、年龄相匹配的健康体检者血清作为对照组。采用ELISA检测所有研究对象血清中LAMP-2抗体水平,对LAMP-2抗体水平与AASV患者ESR、CRP、伯明翰血管炎活动积分(BVAS)、血红蛋白、白蛋白等指标进行相关分析。2组间LAMP.2抗体水平比较采用t检验,LAPM-2抗体水平和ESR、CRP相关性检验采用Spearman相关分析,LAMP-2抗体水平和BVAS积分、血红蛋白、白蛋白相关性检验采用Pearson相关分析。结果①AASV患者血清中LAMP-2抗体水平[(3714±1446)pg/m1]高于对照组[(174±43)pg/m1](t=10.94,P〈0.05),血红蛋白[(99±30)g/L]、白蛋白[(27±5)只,L]明显低于对照组[(138±14)g/L,(44±3)g/L](t=5.27,t=13.04,P均〈0.05)。②AASV患者血清中LAMP-2抗体水平与BVAS积分呈正相关(r=0.6699,P〈0.05),与ESR、ClIP、血红蛋白、白蛋白无相关性(P〉0.05)。结论①LAMP-2抗体可能参与了AASV的发病过程;②LAMP-2抗体能反映AAsv疾病活动性,可作为血管炎活动的血清学临床监测指标。
Objective To explore the clinical significance of serum anti-lysosomal associated membrane protein-2 (LAMP-2) antibody in the pathogenesis of anti-neutrophil cytoplasmic antibodies (ANCA)- associated vasculitis (AASV) by investigating the relationship of its levels and AASV. Methods Sera from twenty patients with AASV and twenty healthy controls were collected. Serum anti-LAMP-2 antibody was detected using commercial enzyme linked immunosorbent assay (ELISA) kits. Anti-LAMP-2 antibody levels between the two groups were assessed using the t test, the correlation between anti-LAMP-2 antibody levels and erythrocyte sedimentation rate (ESR), C-reactive protein (CRP) was assessed by Spearman's rank cor- relation test, the correlation between anti-LAMP-2 antibody levels and the Birmingham vasculitis activity score (BVAS), hemoglobin (Hb), albumin (Alb) was assessed by Pearson's rank correlation test. Results (1) The serum level of anti-LAMP-2 antibody in patients with AASV [(3 714±1 446) pg/ml] was higher than that in the healthy controls [(174±43) pg/ml] (t=10.94, P〈0.05). The serum level of Hb [(99±30) g/L] and Alb [(27±5) g/L] in patients with AASV was lower than that in healthy controls [(138±14) g/L, (44±3) g/L] (t=5.27, t=13.04, P〉0.05). (1) The level of anti-LAMP-2 antibody in AAS-V::was positively correlated with BVAS (r=0.669 9, P〈0.05), and was not found elevated compared with ESR, CRP, Hb and Alb (P〉0.05). Conclusion (1) Anti-LAMP-2 antibody is involved in the pathogenesis of AASV. (2) Anti-LAMP-2 antibody is correlated with the activity of AASV, it may be an indicator of AASV disease activity.
出处
《中华风湿病学杂志》
CAS
CSCD
北大核心
2016年第5期321-324,共4页
Chinese Journal of Rheumatology
关键词
抗体
溶酶体相关膜蛋白2
ANCA相关性小血管炎
Antibody
Lysosomal associated membrane protein-2
Anti-neutrophil cytoplasmic antibodies-associated vasculitis