天津地区番茄褪绿病毒的分子检测与基因组部分序列分析

Molecular Detection and Partial Genome Sequence Analysis of Tomato chlorosis virus in Tianjin

为了确定天津番茄产区是否发生了番茄褪绿病毒病,了解该病毒天津分离物的主要基因是否发生变异,对该地区发生的To CV进行了分子检测,并对该病毒的外壳蛋白(CP)和热激蛋白70类似物(Hsp70h)的核苷酸和氨基酸进行了序列分析。结果表明,天津分离物CP蛋白的核苷酸和氨基酸序列与已报道的具有代表性的不同国家的分离物相似性均在97.3%以上。Hsp70h蛋白的核酸和氨基酸序列与已报道的具有代表性的不同国家的分离物相似性均在97.2%以上。表明CP和Hsp70h蛋白是2个最保守的蛋白。这是首次在分子水平上证明番茄褪绿病毒在天津地区为害。

The purpose of this study was to determine whether the tomato plants in Tianjin was infected by Tomato chlorotic virus（ To CV） and whether variation had occurred in the important genes of To CV. To CV isolated in Tianjin was diagnosis at molecular level. The genome sequence of coat protein and heat shock protein had been cloned and analyzed. The results showed that the homology of the CP isolate in Tianjin was 97. 3% or more with the representative isolates of different countries and regions. The homology of the Hsp70 h isolate in Tianjin was 97. 2%or more with the representative isolates of different countries and regions. It is proved that Hsp70 h and CP protein are the most conserved proteins. This is the first report of Tomato chlorosis virus occurrence in Tianjin.