放线菌对干旱胁迫下黑麦草生长及抗氧化特性的影响

Effects of Actinomycetes on the Growth and Antioxidative Characteristics of Perennial Ryegrass under Drought Stress

采用菌剂接种及盆栽生物实验,研究了干旱缺水条件下放线菌对黑麦草生长和抗氧化特性的影响。结果显示:(1)干旱胁迫下,土壤中接种放线菌显著促进黑麦草的生长,其中根分蘖及根部生物量分别显著增加35.00%和37.47%;(2)接种放线菌后,黑麦草叶片的叶绿素总量和类胡萝卜素含量分别显著增加了12.02%和10.38%;(3)加菌后3种主要的抗氧化酶超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)较不加菌对照均增加,其增率分别为12.72%、2.38%和24.83%,其中SOD和CAT的活性增加显著。研究表明,干旱胁迫下放线菌接种土壤后,提高了黑麦草叶绿素含量和类胡萝卜素含量,增强了黑麦草抗干旱胁迫的抗氧化酶活性,可显著促进黑麦草的生长,增加其生物量。

We investigated the effects of actinomycetes on the growth and antioxidative characteristics of pe- rennial ryegrass under drought stress, using soil inoculating actinomycetes agent and pot experiment. Re- sults showed that： （1）actinomycetes-inoculated treatment significantly promoted the plant growth under drought stress,of which root tiller number and root fresh weight were significantly increased by 35.00% and 37.47 ~, respectively（P^0.05） ;（2）the contents of total chlorophylls and carotenoid were significantly increased by 12.02~ and 10.38~//oo with actinomycetes-inoculated under drought stress; （3）the three main antioxidative enzyme activities of SOD,POD and CAT were increased by 12. 720//oo ,2.38o//oo and 24.83~ ,re- spectively,while SOD and CAT activities showed significantly increased with actinomycetes inoculation. These results indicated that actinomycetes-inoculated in drought soil improved the contents of total chloro- phylls and carotenoid, enhanced the antioxidative characteristics of perennial ryegrass,and thus significant- ly promoted the plant growth by increasing plant biomass.