基于NLRP3炎性体信号通路研究桂枝芍药知母汤治疗痛风性关节炎的作用机制

Research on Mechanism of Guizhi Shaoyao Zhimu Tang in Treatment of Gouty Arthritis Based on NLRP3 Inflammasomes Signaling Pathway

目的:探讨桂枝芍药知母汤(GT)对尿酸钠致痛风性关节炎(GA)模型大鼠关节滑膜组织中炎性信号表达的影响。方法:180只雄性SD大鼠随机分配到3个实验,分别为关节滑膜免疫组化实验,酶联免疫吸附测定(ELISA)实验,蛋白质免疫印迹(Western blot)实验。各实验取大鼠60只,按体重随机分为6组,每组10只,分别为模型组,正常组,GT高、中、低剂量组(4,8,16 g·kg^(-1)),秋水仙碱阳性药组(3×10-4g·kg^(-1))。实验组均ig给药,正常组、模型组给予等容积的蒸馏水,每天1次,连续给药7 d。第5天ig前,大鼠足踝关节注射尿酸钠悬液诱导GA。取大鼠关节滑膜组织,免疫组化检测Nod样受体蛋白3(NLRP3)炎性体的表达,Image-Pro Plus6.0图像分析系统测定平均积分吸光度(IA),Western blot检测凋亡相关斑点样蛋白(ASC),半胱氨酸天冬氨酸酶^(-1)(Caspase^(-1))信号衔接蛋白表达,ELISA测定炎性因子白细胞介素^(-1)β(IL^(-1)β),白细胞介素-6(IL-6),肿瘤坏死因子-α(TNF-α),核因子-κB(NF-κB)表达水平。结果:造模72 h后,与正常组比较,模型组大鼠关节滑膜组织中NLRP3,ASC,Caspase^(-1),L^(-1)β,IL-6,TNF-α,NF-κB表达明显升高(P<0.05),Caspase^(-1)2表达明显降低(P<0.05);与模型组比较,GT高、中剂量组NLRP3,ASC,GT各剂量组Caspase^(-1)表达水平均显著降低(P<0.05),Caspase^(-1)2表达明显升高(P<0.05),GT各组IL^(-1)β,IL-6,TNF-α,NF-κB表达均明显降低(P<0.05)。结论:GT治疗GA的作用机制可能与降低NLRP3,ASC,Caspase^(-1)表达,抑制IL^(-1)β分化成熟及NF-κB活化,降低NLRP3炎性体信号通路炎性因子表达有关。

Objective： To investigate the effect of Guizhi Shaoyao Zhimu Tang（ GT） on inflammatory signal expression in synovial tissues of joint of rats with monosodium urate crystal-induced gouty arthritis（ GA）.Method： Totally 180 male SD rats were random Ly divided into 3 experiments [the Immunohistochemistry,enzyme linked immunosorbent assay（ ELISA）,Western blot experiment] with 60 rats each experiment divided into 6 groups with 10 rats each group according to weight. The high,medium and low dose group of GT（ 4,8,16 g·kg-1） and colchicine group（ 3 × 10- 4g·kg-1） were treated with medicine by gastric administration,the normal and model group were given equal volume of distilled water. Medicine or distilled water was given once daily for seven consecutive days throughout the experiment. On the fifth day, GA model was made by injection of monosodium urate in the ankle joint cavity of rat. The synovial tissues of the rats were taken and expression of nodlike receptor protein 3（ NLRP3） inflammasomes were detected with immunohistochemistry and integrated absorbance（ IA） was measuring with Image-Pro Plus 6. 0 analysis system. The expression levels of apoptosisassociated speck-like protein containing a CARD（ ASC）,Caspase-1 signaling proteins were detected by Western blot. Expression of inflammatory cytokine such as interleukin-1 beta（ IL-1β） and interleukin-6（ IL-6）,tumor necrosis factor alpha（ TNF-α）,nuclear factor-κB（ NF-κB） were detected with ELISA. Result： Compared with normal group after 72 hours,the expression of NLRP3 inflammasomes,ASC,Caspase-1 and IL-1β,IL-6,TNF-α,NF-κB in synovial tissues of the joint of GA rats significantly increased（ P 〈0. 05）, whereas Caspase-12 significantly decreased（ P〈 0. 05）. the expression of NLRP3 inflammasomes, ASC in medium and high dose group of GT and Caspase-1 in all group of GT significantly decreased than the model group（ P 0. 05）,whereas Caspase-12 increased（ P 0. 05） and there was significant decreasing change in IL-1β,IL-6,TNF-α,NF-κB in all dose group of GT. Conclusion： The acion mechanism of GT on GA in rats is related to decreasing the expression levels of NLRP3,ASC and Caspase-1 and increasing the expression of Caspase-12,and accordingly inhibiting the maturation of IL-1β and the activation of NF-κB,reducing the expression of inflammatory factors in NLRP3 inflammasomes signaling pathway.