13个中国葡萄优新品种的分子身份证构建

Molecular ID Establishment of 13 Chinese Newly-developed Grape Cultivars

以近年来选育的13个葡萄优新品种为试材,进行亲缘关系分析,构建分子身份证,为中国葡萄品种鉴定和新品种保护提供一定的参考。利用国际通用的9对引物对供试葡萄品种进行SSR分析,并根据引物对不同品种扩增条带分子量的大小进行赋值编码。结果表明,该9对引物在供试葡萄品种间共检测出48个等位基因,每对引物平均检测到等位基因数为5.3个;期望杂合度的范围在0.435-0.811之间;多态性信息含量变幅为0.401-0.784。同时基于SSR分析结果得到编码各异的品种分子身份证,该分子编码可以将13个葡萄品种进行有效区分。聚类分析显示13个品种根据亲缘关系远近聚为不同类别。

Using 13 newly-developed grape cultivars as experimental materials,the phylogenetic relationship among them were analyzed and their molecular IDs were established,which aimed to provide the references for identifying the grape cultivar and protecting the new ones. SSR analysis was carried out on the tested grape cultivars with the international commonly-used 9 pairs of primers,and the codes of each cultivar was assigned according to the molecular weight of the amplified band of different cultivar by the primer. The results showed ：a total of 48 alleles and an average of 5.3 alleles per primer were detected by 9 pairs of primers. The expected heterozygosity varied between 0.435-0.811,and the information content of polymorphism ranged from 0.401 to 0.784. After alleles assignment and coded,the molecular IDs of cultivars were established,by which the 13 grape cultivars can be identified. According to the clustering analysis,13 cultivars showed clear separations due to their different parents.