红提葡萄内生细菌的分离鉴定及灰霉病拮抗菌的筛选

Isolation and Identification of Endophytic Bacteria in Red Grape,and Screening of Antagonistic Bacteria Against Botrytis cinerea

旨在从新疆红提葡萄的不同部位中,分离筛选出对葡萄灰霉病具有较强拮抗作用的内生细菌。采用组织分离法对红提葡萄不同部位的内生细菌进行分离,同时进行16S r DNA序列测定及同源性分析,并采用平板对峙法和滤纸片法进行筛选,研究其对葡萄灰霉菌的抑制作用。结果表明,在分离得到的18株内生细菌中,有5株具有良好的拮抗效果,分别为NR4-1、NR5-1、NG4-1、NP1-1和PG1,抑菌率可达68.7%。

This experiment aims to separate the endophytic bacteria,which have the strongly antagonistic effect on Botrytis cinerea,from different parts of red grape in Xinjiang. The tissue isolation was adopted to separate the endophytic bacteria from different parts of red grape,and concurrently 16 S r DNA sequence determination and homology analysis were carried out. Meanwhile,the antagonistic effect of endophytic bacteria on B. cinerea was analyzed by confrontation culture method and disc diffusion assay method. The results showed that 5 of 18 strains of endophytic bacteria had solid antagonistic effect,they were NR4-1,NR5-1,NG4-1,NP1-1,and PG1 respectively,and the bacteriostasis rate reached 68.7%.