Gas-6蛋白对小鼠急性肺损伤保护作用的研究

The protective effect of Gas-6 protein on murine acute lung injury

目的研究外源性Gas-6蛋白对脂多糖(LPS)诱发小鼠急性肺损伤的保护作用。方法将Balb/c小鼠随机分为空白对照组、Gas-6蛋白对照组、LPS处理组、Gas-6蛋白保护组。LPS处理组、Gas-6蛋白保护组分别通过腹腔注射LPS诱导急性肺损伤模型,其余组以等量生理盐水作为对照。而后Gas-6蛋白对照组及Gas-6蛋白保护组立刻通过尾静脉注入Gas-6蛋白,其余两组以等量生理盐水作为对照。LPS/生理盐水注射12 h后,取小鼠血清及肺组织。HE染色观察小鼠肺组织病理学改变、测定肺组织病理学评分、肺组织湿/干质量比、检测血清中肿瘤坏死因子-α(TNF-α)和白介素-6(IL-6)水平以及肺组织中TNF-αmRNA、IL-6 mRNA的表达情况。结果肺组织病理学检查显示:与空白对照组比较,LPS处理组小鼠肺水肿、肺组织充血、中性粒细胞浸润、炎性渗出较明显,而Gas-6蛋白可减轻上述改变。LPS处理组小鼠肺组织湿/干质量比、肺组织病理学评分、血清中TNF-α、IL-6水平及肺组织中TNF-αmRNA、IL-6 mRNA的表达较空白对照组升高,差异有统计学意义(P<0.05)。而Gas-6蛋白保护组上述指标较LPS处理组均有所下降,差异有统计学意义(P<0.05)。结论 Gas-6蛋白对LPS所诱导的急性肺损伤小鼠有保护作用。

Objective To investigate the effect of exogenous Gas-6 protein on LPS-induced acute lung injury mice. Methods Balb/c mice were randomly divided into control group, Gas-6 protein control group, LPS-treated group and Gas-6 protein protection group. LPS was administered to induce murine acute lung injury model by intraperito- neal injection in LPS-treated group and Gas-6 protein protection group, while the other groups used normal saline. Then Gas-6 protein was intravenously injected in Gas-6 protein control group and Gas-6 protein protection group at once, while the other groups used normal saline. 12 hours after LPS/normal saline injection, serum and lung tissue of mice were obtained. HE staining was used to observe pathological changes in the lung tissue of mice. The lung pathological score, lung tissue wet-to-dry weight ratio, the levels of TNF-o~ and IL-6 in serum, the expression of TNF-ot mRNA and IL-6 mRNA in lung tissue were analyzed at the same time. Results The pathological results showed that the degrees of pulmonary edema, lung congestion, neutrophil infiltration, inflammatory exudate of the LPS-induced group were more serious than the control group. However, the Gas-6 protein could reduce the chan- ges. Compared with the control group, the lung pathological score, lung tissue wet-to-dry weight ratio, the levels of TNF-α and IL-6 in serum, the expression of TNF-α mRNA and IL-6 mRNA in lung tissue of the LPS-induced group were significantly increased （P 〈 0.05 ）. However, the index of the Gas-6 protein protection group was lower than the LPS-induced group, and the difference was statistically significant（P 〈0. 05 ）. Conclusion Gas-6 protein has protective effect on acute lung injury induced by LPS in mice.