犬细小病毒新2b型分离株的分离鉴定

Isolation and identification of new canine parvovirus type 2b isolate

为了更好的了解我国东北地区犬细小病毒的流行情况,采用F81细胞从来自长春某宠物医院疑似患有肠炎的犬粪便样品中分离出1株病毒,经形态学、血清学、动物回归试验和分子生物学鉴定,分离的病毒为犬细小病毒new CPV-2b型,命名为CPV JL13-1。对该病毒主要结构蛋白VP2基因进行克隆测序和基因进化分析表明,CPV JL13-1分离株VP2基因与GenBank上提交的其他41株犬细小病毒株核苷酸和氨基酸均有较高的同源性,分别为98.6%-99.5%和97.6%-99.5%,其中核苷酸和氨基酸同源性最高的均为B-2004株。本研究为犬细小病毒分子流行病学调查和疫苗的研究奠定基础。

To better understand the prevalence of canine parvovirus（CPV）in northeast of China,we isolated a virus from dog showing clinical and pathological signs of enteritis.This virus strain,named as CPV JL13-1,was identified as new CPV-2bfrom results of morphology,serologic test,artificial infection of Beagle dogs and molecular biology.Cloning and phylogenetic analysis of the capsid protein VP2 gene,and results indicated CPV JL13-1 VP2 gene shared higher homology with other 41 CPV isolations in GenBank.The homology of nucleotide and deduced amino acids were 98.6%-99.5% and 97.6%-99.5%,respectively.The highest degree of nucleotide homology with B-2004 strain was 99.5%,and the highest degree of deduced amino acids homology with other eight isolations,including B-2004 isolate,were 99.5%.The obtained VP2 gene sequence has been submitted to GenBank with the accession number KR058183.This study provided the basic evidence for molecular epidemiology investigation and research of vaccines of CPV.