玉米1-吡咯啉-5-羧酸合成酶在非生物胁迫下的表达分析

Differential exprssion of maize delta-1-pyrroline-5-carboxylate synthase(P5CS)gene family members under different abiotic stress

脯氨酸在植物渗透调节中起举足轻重的作用,1-吡咯啉-5-羧酸合成酶(P5CS)是脯氨酸的谷氨酸合成途径中的关键酶,为了研究玉米P5CS基因家族与玉米抗逆性的关系,对玉米幼苗进行了非生物胁迫(干旱、低温、盐胁迫)处理,利用半定量RT-PCR技术分析了ZMP5CS基因家族在不同胁迫条件下的表达特性。结果表明,ZMP5CS基因家族中除ZM02G27230以外的基因不论地上部叶还是地下部根均被干旱胁迫诱导上调表达,但各成员之间其表达量最高点出现的时间不同;相同处理同一基因相同的处理时间点地上部与地下部基因表达量存在差异,在干旱胁迫下ZM08G14210的表达量最高,在盐胁迫下ZM08G31890的表达量最高,在低温胁迫下ZM06G25580的表达量最高,说明ZMP5CS基因家族表达受非生物胁迫的诱导。

Proline plays an important role in plant osmotic regulation, Delta-l-pyrroline-5-carboxylate synthase （ P5CS ） is a key enzyme in proline of glutamic acid synthesis pathway. In order to study the relationship between P5CS gene families and abiotie stress in maize, the maize seedings were treated with ahiotie stress （ drought, low temperature and salt stress ） . The expression levels of ZMPSCS in maize （ leaf, root ） under different stresses were analyzed by semi-quantitative t/T-PCR. The results showed that the excepted ZMO2G27230 in the ZMP5CS gene family both up-regulated in the leaf and root by drought stress, but its expression among each member peak appeared in different time. The same treatment of the same gene time point of leaf and root, gene expression was different. Under drought stress, ZMOSG14210 expression quantity was the highest. Under salt stress, ZMOSG31890 expression quantity was the highest. Under low temperature stress, ZMO6G25580 expression quantity was the highest. The results indicated that the ZMP5CS expression was induced by abiotie stress.