基于荷正电聚合物探针的共振光散射法DNA杂交检测

Positive Charged Polymer as a Probe for the Determination of DNA Hybridization by Resonance Lighting Scattering

荷正电高分子聚合物可以通过静电作用与DNA寡核苷酸序列进行结合,其与单链DNA和双链DNA结合后有不同的共振光散射（RLS）信号.基于此,设计了一种杂交DNA检测探针,实现了完全互补序列与单碱基错配序列及非互补碱基序列的区分,并建立了一种简单、快速、免标记的DNA杂交检测方法.在最佳条件下,在λ=470nm处,光散射强度达到最大,并且与目标DNA浓度在5.0～500nmol/L范围内呈线性,检出限为2nmol/L.

In this paper, we applied a positive-charged tion, proposed a simple and speed based on the different resonance li and established a new label-free m y g e polymer for the determination of DNA hybridiza- assay for specific oligonucleo ht scattering （RLS） signals o thod for the determination of tide sequences and single-base mismatch f polymer/ssDNA and polymer/dsDNA, DNA hybridization. Under the optimum conditions, the enhanced RLS intensity at 470 nm was proportional to the concentration of target DNA over the range of 5.0 to 500 nmol/L, with the limit of determination about 2 nmol/L.