天麻素对脑缺血再灌注损伤大鼠相关基因mRNA表达的影响

Effect of Gastrodin on related genes mRNA expressions in cerebral ischemia-reperfusion rats

目的观察天麻素对脑缺血再灌注大鼠脑组织中缺氧诱导因子-1α(HIF-1α)、血管内皮生长因子(VEGF)等基因mRNA表达的影响。方法灌胃不同剂量天麻素7 d,采用线栓法制备大鼠大脑中动脉局灶性脑缺血(2 h)再灌注损伤模型,造模成功后继续给药7 d。采用实时荧光定量PCR(Real Time RT-PCR)法检测缺血半暗带脑组织中HIF-1α、VEGF、BDNF、MMP2、e NOS、PPARα及SOD1 mRNA的表达。结果天麻素可上调HIF-1α、VEGF、BDNF、MMP2、e NOS、PPARα、SOD1 mRNA的表达(P<0.05),下调MMP2 mRNA的表达(P<0.05)。结论天麻素可通过上调大鼠脑缺血半暗带HIF-1α、VEGF、BDNF、MMP2、e NOS、PPARα、SOD1 mRNA的表达,下调MMP2 mRNA的表达,产生抗局灶性脑缺血再灌注损伤的作用。

Objective To explore the effects of Gastrodin（ Gas） on the mRNA expressions of hypoxia inducible factor- 1α（ HIF- 1α）,vascular endothelial growth factor（ VEGF） and other genes in the ischemic penumbra of rats injured by ischemia（ 2 h）- reperfusion（ I / R）. Methods Rats were pretreated with Gas for 7 days followed by the focal cerebral I / R and then Gas was withdrawn 7 days after the I / R surgery. The mRNA expressions of HIF- 1α,VEGF,brain derived neurotrophic factor（ BDNF）,matrix metalloproteinase- 2（ MMP2）,endothelial nitric oxide synthase（ e NOS）,peroxisome proliferators activated receptors（ PPARα） and superoxide dismutase- 1（ SOD1） in the ischemic penumbra of rats were detected by Real Time RT- PCR. Results The expressions of HIF- 1α,VEGF,BDNF,MMP2,e NOS,PPARα and SOD1 mRNA in Gas group increased obviously（ P 〈 0. 05）,while the expression of MMP2 mRNA in Gas group decreased obviously（ P 〈 0. 05）.Conclusion Gas has protective effects on I / R- injured rat brain by up- regulating the expressions of HIF- 1α,VEGF,BDNF,e NOS,PPARα and SOD1 and down- regulating the expression of MMP2.