三乙胺对HBE细胞的氧化损伤作用

Triethylamine-induced oxidative damage to human bronchial epithelial cells

目的探讨三乙胺对永生化人支气管上皮细胞(HBE)的氧化损伤作用。方法体外培养HBE细胞,在6个不同染毒浓度(0、5、7.5、10、15、20mmol/L)和4个不同染毒时间段(6、12、24、48h)的条件下染毒HBE细胞,CCK-8试剂盒检测三乙胺对HBE细胞存活率的影响;收集细胞后装载探针检测细胞活性氧(ROS)的含量;同时通过单细胞凝胶电泳试验(SCGE)检测细胞DNA链断裂情况。结果在相同染毒时间段内,HBE细胞的存活率随着染毒浓度的增加而下降,各浓度组与对照组的差异有统计学意义(P<0.05),且呈明显的剂量-效应关系;随着染毒浓度的增加,HBE细胞ROS含量明显上升,与相同染毒时间段对照组比较,差异有统计学意义(P<0.05);SCGE实验结果表明,各组HBE细胞Olive尾距随染毒浓度增加明显增加,呈现明显的剂量—反应关系,与相同染毒时间段对照组比较,差异有统计学意义(P<0.05)。结论三乙胺可降低HBE细胞存活率,诱导HBE细胞产生自由基从而引起DNA损伤。

Objective To determine the oxidative damage of Triethylamine（TEA）on Human bronchial epithelial（HBE）cells.Methods CCK-8assay was used to test the cytotoxicity of TEA on HBE.The HBE cells were exposed to 0,5,7.5,10,15,and 20mmol/L of TEA for 6h,12 h,24hand 48 h.The contents of reactive oxygen species（ROS）in the HBE cells were determined after collecting cells loaded probe method.Single cell gel electrophoresis（SCGE）were used to detect the DNA damage in the HBE cells.Results In the same exposure time periods with the increase of TEA,the viability of HBE cells decreased compare to that in the control（P〈0.05）and significant dose-effect relationships were found.The intracellular ROS of the HBE cells was significantly increased compare to the control（P〈0.05）.The results of SCGE indicated that TEA induced DNA strand break in the HBE cells.The olive tail moment（OTM）increased with the increase of TEA concentrations and there were significant differences compare to the control（P〈0.05）.Conclusions TEA can reduce the viability of HBE cells,induced HBE cells to produce more ROS that cause DNA damage.