附子不同炮制品三种单酯型生物碱的含量差异研究

Determination of Three Kind of Diester Diterpenoid Alkaloids in different Radix Aconiti Lateralis Praeparata

目的研究乌头碱、新乌头碱和次乌头碱在不同主产地不同附子炮制品中的含量变化,为附子中药的行业质量标准的建立提供依据。方法采用Alltimate C18色谱柱（250 mm×4.6 mm,5μm）;以40 mmol·L-1醋酸铵（氨水调p H=10.00）-乙腈为流动相梯度洗脱;流速1.0 ml·min-1;检测波长240 nm。结果三种单酯型生物碱新乌头碱、乌头碱、次乌头碱均得到良好的分离,回归方程分别为：Y=15.952X-16.118 R2=0.9997;Y=15.906X-2.6445 R2=0.9998;Y=13.218X-23.044 R2=0.9999。三者线性范围是2.5~250μg,2.2~220μg,2.6~260μg。结论该方法的精密度、重复性和稳定性良好,可用于附子炮制品的鉴别和内在质量控制。

Objective To study the aconitine,mesaconitine and hypaconitine in different Radix Aconiti Lateralis Praeparata which in different main place of origin,and provide the basis for the establishment of quality standards. Methods A Alltimate C18column（ 250 mm × 4. 6 mm,5μm） was used,and ammonium acetate solution- acetonitrile as the mobile phase,with a flow rate of 1. 0 m L·min- 1and a detection wavelength of 240 nm. Results Mesaconitine,aconitine and hypaconitine,were separated.The linear ranges of mesaconitine,aconitine,hypaconitine were respectively 2. 5 ~ 250 μg（ R2= 0. 9997）,2. 2 ~ 220 μg（ R2=0. 9998）,2. 6 ~ 260 μg（ R2= 0. 9999）. Conclusion The method is accurate,repeatable and reliable,it can be used in the identification and quality control of A. carmichaeli.