小麦专用复方抗旱型浸种剂和包衣剂对小麦幼苗SOD、POD和CAT同工酶表达的影响

The Effects of Compound Anti-drought Seed Soaking Agent and Seed Coating Agent on SOD,POD and CAT Isozyme Expression in Wheat Seedlings

为从抗氧化酶同工酶表达方面了解小麦专用复方抗旱型浸种剂和包衣剂对小麦抗旱性的调节作用,以小麦品种临麦2号为材料,采用盆栽试验法,研究了小麦专用复方抗旱型浸种剂和包衣剂处理种子对干旱胁迫下小麦幼苗超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)同工酶表达的影响。结果表明,干旱胁迫下,小麦幼苗Mn-SOD和Fe-SOD表达量(活性)高于对照,而Cu/Zn-SOD同功酶表达量(活性)降低。与对照相比,干旱胁迫下,POD同工酶中的Rf 0.05、Rf 0.13、Rf 0.22和Rf 0.61条带表达量降低,抗旱型包衣剂和浸种剂处理种子均提高干旱胁迫下Rf 0.05、Rf 0.13和Rf 0.61条带的表达量,而普通包衣剂处理增加了Rf 0.18、Rf 0.22条带的表达量。干旱胁迫降低了CAT同功酶Rf 0.04、Rf 0.76条带的表达量,其中Rf 0.04表达量降幅较大,而抗旱型包衣剂和浸种剂处理种子均提高干旱胁迫下这两个条带的表达量,尤其是Rf 0.04的表达量显著超过对照。说明抗旱型包衣剂和浸种剂能够调控小麦幼苗抗氧化酶同工酶的表达,进而增强植株的抗氧化能力,提高小麦的抗旱性。

The effects of anti-drought seed soaking agent and seed coating agent on the oxidation re- duction system isozyme expression were explored with wheat variety Linmai 2 under drought stress by controlling soil moisture in plant pots. The results showed that the Mn-SOD and Fe-SOD isozyme a- mount （activity） were higher than control; Cu/Zn-SOD isozyme amount （activity） was lower than control under drought stress; The POD isozyme expression results showed that the amount of band Rf 0.05, Rf 0.13, Rf 0.22 and Rf 0.61 was less than contrast under drought treatment; The amount of band Rf 0.05, Rf 0.13 and Rf 0.61 were exceeded over control by the treatments of ＂ anti-drought seed soaking agent＂ and ＂anti-drought seed coating agent＂ under drought stress; The new bands of Rf 0.18 and Rf 0.22 appeared under ＂drought stress＂ and ＂anti-drought seed soaking with drought stress＂ . The CAT expression showed that the amounts of band Rf 0.04 and Rf 0.76 were lower than control under ＂drought stress＂ treatment; The amounts of band Rf 0. 04 and Rf 0. 76. especially Rf 0.04 were significantly higher than control under the ＂anti-drought seed soaking with drought stress＂ and ＂anti-drought seed coating with drought stress＂ treatments. In summary, the seeds trea- ted by drought-resistant seed soaking agent or seed coating agent could enhance isozyme expression by upgrading oxidation reduction system under drought stress, which increased drought-resistant and an- tioxidant ability of wheat seedling.