应用活细胞RNA检测纳米技术检测乳腺上皮细胞MCF-10A及乳腺癌MCF-7细胞的miR-142-3p表达

Using MicroRNA Conjugated Nanoparticles for the Detection of miR-142-3p in Live MCF-10A and MCF-7 Cells

传统的核酸检测技术如放射性核素、荧光、化学修饰的探针以及核酸扩增等技术无法检测活细胞中核酸的表达量。而活细胞RNA纳米检测技术和传统的检测技术相比,利用纳米金颗粒为探针能对活细胞进行检测,实验步骤更为简单,可以在自然的、无扩增的条件下观察RNA,这可真实地反应基因表达与表型之间的关系。miRNA是一类非编码RNA,其长度为20~24个碱基,在生命活动中起重要的作用。本文应用活细胞RNA检测纳米技术结合荧光定量PCR分别检测正常的乳腺上皮细胞系及乳腺上皮癌细胞系中内源性miR-142-3p的表达,发现乳腺癌细胞系内源性miR-142-3p的表达显著高于正常乳腺上皮细胞系中miR-142-3p的表达,结果提示miR-142-3p可能在乳腺癌细胞发生发展中起到调控作用。

The procedures using nanotechnology with classical methods, as RNA can be observed for RNA detection in living cells is simpler compared under the natural conditions without amplification to faithfully reflect the correlation between gene expression and phenotype, miRNA is a class of non-coding RNAs which consists of 20 - 40 bases with an important role in determining life activities. In this study, we detected the expression level of endogenous miR-142-3p in normal mammary epithelial and mammary carcinoma cells in living conditions. The results showed that the miR-142-3p expression was significantly higher in mammary epithelial cells carcinoma than in normal mammary epithelial cells. It indicated that miR-142-3p might play a regulatory role in the development of breast cancer cells.