新铁炮百合与东方百合杂种成花诱导关键时期的确定

Determination of the Critical Period of Floral Induction for Hybrid Plants Between Lilium × formolongi and Oriental Hybrid Lily

试验材料是从新铁炮百合（Lilium×formolongi）与东方百合‘索邦’（Oriental hybrid‘Sorbonne’）的杂交苗中筛选出的优良株系。以该株系组培扩繁的试管鳞茎为材料,通过石蜡切片观察从营养生长到生殖生长阶段的茎尖解剖结构;通过交互迁光试验初步确定光周期感受态及限界性诱导光周期;在不同光周期下,通过qRT-PCR技术测定分析了杂种苗不同组织和发育时期成花诱导相关基因CONSTANS（CO）和FLOWERING LOCUS T（FT）的表达。结合形态特征、解剖结构和基因表达状况,最终确定了该百合杂种苗成花诱导关键时期：在6～8片基生叶时开始进入光周期感受态,在2～4个节间时开始进入成花诱导期,限界性诱导光周期为28 d。

The excellent strains were selected in the hybrids from Lilium × formolongi and Oriental hybrid ‘Sorbonne＇ and the hybrid bulblets propagated by tissue culture were used as materials. The anatomy structure of the meristems was observed by paraffin section. The experiment that the materials with different numbers of basal leaves in the short day were moved into the long day preliminarily confirmed the age of photoperiod. Another experiment that the materials in the long day were moved into the short day determined the circumscription of photoperiod. Under different photoperiods, qRT-PCR were used to analysis the expression of CO, FT in different tissues in different growth development stages. The critical periods of the flower induction were determined by the combining of the morphological features, anatomy structure and gene expression. Comprehensively, the hybrids began to enter into the photoperiod competent in the growth development of 6 - 8 leaves and the stage of flowering induction in the period of 2 - 4 internodes. Moreover, the circumscription of photoperiod was 28 days.