砀山酥梨和秋白梨酚类物质UPLC–PDA–MS/MS–ESI分析

UPLC–PDA–MS/MS–ESI Analysis of Phenolic Compounds in Fruits of Dangshan Suli and Qiubaili Pears(Pyrus bretschneideri)

采用超高效液相色谱—二极管阵列—串联四级杆质谱联用法(UPLC–PDA–MS/MS–ESI)对砀山酥梨和秋白梨果皮、果肉和果心(不含种子)中的酚类物质进行了分析。结果表明,两个品种检出42种酚类组分。大多数酚类物质在果皮中含量最高,仅绿原酸和Z–咖啡酰奎尼酸在果心最高。果皮中的酚类物质种类最多,砀山酥梨果皮中含有5大类42种;秋白梨果皮中含有4大类30种,砀山酥梨比秋白梨多7种黄酮类组分和3种槲皮素糖苷类物质。在果心和果肉中未检出黄烷醇类、黄酮醇类和黄酮类物质。在砀山酥梨果皮中鉴定出其他梨果实中尚未见有报道的芹菜素7–O–芸香糖苷,在砀山酥梨和秋白梨果皮中鉴定出原花青素B1和B2两种原花青素二聚体和原花青素C1及暂命名的C-1、C-2和C-3等4种原花青素三聚体。

Polyphenolic profiles in peel, pulp and core （without seeds） of two kinds of Pyrus bretschneideri Rehd., one is Dangshan Suli and the other is Qiubaili, were respectively studied by UPLC - PDA - MS/MS - ESI. The results showed that forty-two kinds of phenolic component of the two kinds of Pyrus bretschneideri Rehd. were identified. Most kinds of the phenolic component contained in peel were more than those contained in pulp or core. But only 5-caffeoylquinic acid （chlorogenic acid） and Z-caffeoylquinic acid focused on the fruit core tissue. The peel tissue contained more types of phenolic compounds. In peel of Dangshan Suli, there were 42 kinds of phenolic substances belong to 5 groups. And in peel of Qiubaili, there were 30 kinds belong to 4 groups. Phenolics of flavanols, flavonols and flavones were not detected in the fruit core tissues or the pulp tissues. The peel＇s phenolic compounds were further compared, the Dangshan Suli＇s have 7 more kinds of flavones glycosides and 3 more kinds of quercetin glycosides than Qiubaili＇s. The apigenin 7-O rutinoside was first identified from Dangshan Suli peel by UPLC - PDA - MS/MS - ESI. No such kind of report was seen for other kinds of pear fruit tissues. B 1, B2, C1, C-1, C-2 and C-3 were also identified tentatively from the Dangshan Suli and Qiubaili peel for the first time.