体外受精-胚胎移植周期中单原核(1PN)胚胎的研究进展

Research advance in embryos derived from monopronucleated zygotes in IVF/ICSI cycles

在体外受精-胚胎移植(IVF-ET)中,常常有单原核(1PN)胚胎出现。这类胚胎最终发展为非整倍体的比例偏高,胚胎发育潜能及妊娠结局极差。本文对近年来国内外有关1PN胚胎的研究进行总结,认为1PN胚胎的形成可能有多种机制,但尚无明确定论。因1PN胚胎的染色体组成多异常,多数研究不建议移植1PN胚胎;然而,国内外也有不少研究已证实,1PN胚胎中染色体正常者也占有一定比例,尤其是IVF-1PN胚胎,可能是正常受精的胚胎,移植IVF-1PN胚胎可获得健康的子代个体。因此,在临床工作中,对于那些无可利用胚胎的患者,在充分告知患者知情同意后,选择IVF-1PN胚胎进行移植不失为一个选择。

In the procedure of human assisted reproduction,embryos derived from monopronucleated zygotes which assume a definite proportion of the numerous morphological normalities. These oocytes could develop into aneuploidy to a scale and affect the embryonic development and pregnancy outcomes consecutively. Whether the embryos derived from monopronucleated zygotes could be transfered in the process of assisted reproduction has not been determined. The review is focused on the discoveries about the single pronucleated zygotes overall the world during the past decades and found that a group of factors have an association with its formation mechanism. The majority of the studies suggested that embryos developed from 1PN zygotes should be discarded for any reproductive purpose as their chromosome constitution are more likely abnormal. Both domestic and foreign researchers, however, have confirmed that the monopronucleated zygotes may fertilize normally, especially IVF-1PN, and develop into a healthy baby. According to this point of view,transplatation of single pronucleated embryos in IVF cycles may be a good option for the couples who obtained none good quality embryos, after they are given fully informed consent.