STAT4基因多态性与乙型肝炎病毒感染相关性肝细胞癌易感性的关系

Relationship between Polymorphisms of STAT4 and the Susceptibility to Hepatocellular Carcinoma Associated with Hepatitis B Virus

目的分析信号传导与转录激活因子4（STAT4）基因多态性与中国人群肝细胞癌（HCC）易感性的关系。方法 154例乙型肝炎病毒（HBV）感染相关HCC为肝癌组,135例性别及年龄匹配的HBs Ag阳性患者为非肝癌组。采用聚合酶链反应-限制性片段长度多态性法（PCR-RFLP）,检测STAT4 rs7574865位点的基因型,并经测序分析验证。Logistic回归分析比较不同基因型与HCC易感风险的关系。结果 STAT4 rs7574865位点3种基因型TT、GT、和GG型在肝癌组的分布频率分别是5.8%（9/154）、37.0%（57/154）和57.1%（88/154）,在非肝癌组分别是8.9%（12/135）、43.7%（59/135）和47.4%（64/135）。各基因型在两组间差异无统计学意义（P〉0.05）;以TT基因型作参照,携带rs7574865 GG型的个体HCC患病风险差异无统计学意义（OR=1.833,95%CI=0.729~4.611,P=0.221）。结论STAT4 rs7574865多态性位点可能与中国人群HBV感染相关HCC易感性无明显相关性。

Objective To investigate the relationship between single nucleotide polymorphism（ SNP） of STAT4 and hepatocellular carcinoma（ HCC） susceptibility in chinese population. Methods 154 patients with hepatitis B virus（ HBV）-related HCC and 135 age-and gender-matched non-HCC controls infected with HBV were enrolled. The genotypes of STAT4 rs7574865 were detected by polymerase chain reaction based restriction fragment length polymorphism（ PCR-RFLP） and verified by sequencing analysis. Logistic regression model was used to analyze the relationship between different genotypes and their susceptibility to HCC. Results The frequencies of genotypes TT,GT and GG on STAT4 rs7574865 were 5. 8%（ 9 /154）,37. 0%（ 57 /154）,57. 1%（ 88 /154） in the HCC group,and 8. 9%（ 12 /135）,43. 7%（ 59 /135）,47. 4%（ 64 /135） in non-HCC group,respectively,showing no statistical significance（ P 0. 05）. Compared with TT genotype,there was no significant association at rs7574865 GG in the susceptibility to HCC（ OR = 1. 833,95% CI = 0. 729 ~ 4. 611,P = 0. 221）. Conclusion The polymorphism of STAT4rs7574865 is not associated with susceptibility to HCC in chinese population.