ERCC1基因多态与铂类联合方案治疗肺鳞癌患者疗效的关系

Association between Polymorphisms of ERCC1 and Efficacy of Platinum-based Chemotherapy for Lung Squamous Carcinoma

目的研究ERCC1基因C8092A及第118位密码子多态性与肺鳞癌患者铂类联合化疗疗效的关系。方法采用聚合酶链反应一限制性片段长度多态性(polymerase chain reaction—based restriction fragment length polymorphism,PCR—RFLP)方法,对80例接受铂类联合方案治疗的肺鳞癌患者的ERCC1基因C8092A及第118位密码子多态性进行检测,分析其多态性与铂类治疗肺鳞癌疗效的相关性。结果 C8092A、第118位密码子野生型患者铂类化疗有效率与非野生型患者比较无明显差别(54.5%vs 36.2%,P=0.13,37.0%vs 43.4%,P=0.58),C8092A、第118位密码子多态性联合分析结果显示:ERCC1两个位点同为CC基因型患者疗效与其他基因型患者疗效比较无统计学差异。C8092A、第118位密码子野生型患者与非野生型患者的无进展生存期(progression free survival,PFS)比较无统计学差异(4个月vs 5个月,P=0.34,5个月vs 5个月,P=0.49)。Cox回归多因素分析显示:ERCC1 C8092A多态性(HR=0.910,P=0.733)、第118位密码子多态性(HR=1.101,P=0.707)均不是肺鳞癌患者PFS的独立预后因素。结论 ERCC1 C8092A、第l18位密码子多态性与肺鳞癌患者铂类联合治疗疗效可能无相关性。ERCC1 C8092A、第l18位密码子多态性与中位PFS也无显著相关。

Objective To assess the association between single nucleotide polymorphisms of C8092 A and codon118 in ERCC1 and efficacy of platinum-based chemotherapy for lung squamous carcinoma. Methods Single nucleotide polymorphisms of C8092 A and codon118 in ERCC1 of 80 patients with lung squamous carcinoma were assessed by PCR-RFLP（ Polymerase chain reaction-based restriction fragment length polymorphism）. Correlation of ERCC1 gene polymorphism and efficacy of platinumbased chemotherapy for lung squamous carcinoma were analyzed. Results There was no significant difference in response rate of patients carrying with wild type compared with non-wild type in C8092 A and C118T（ 54. 5% vs 36. 2%,P = 0. 13,37. 0% vs43. 4%,P = 0. 58）. There was no difference in progression free survival between patients carrying with wild type and non-wild type in C8092 Aand C118T（ 4 months vs 5 months,P = 0. 34,5 months vs 5 months,P = 0. 49）. Multivariate analysis of cox proportional hazard model showed that ERCC1 C8092A（ Hazard ratio = 0. 910,P = 0. 733） and codon 118 polymorphism（ Hazard ratio = 1. 101,P = 0. 707） were not independently associated with the prognosis. Conclusion There may be no association between single nucleotide polymorphisms of C8092 A and codon118 in ERCC1 and efficacy of platinum-based chemotherapy for lung squamous carcinoma. Single nucleotide polymorphisms of C8092 A and codon118 in ERCC1 and progression free survival also have no significant correlation.