藏族药榜嘎质量标准分析

Research on Quality Standard of Ponka

目的：建立榜嘎药材的质量控制方法和标准,为有效控制该药材的质量提供参考。方法：参照《中国药典》2015年版四部通则相关方法,对榜嘎药材的水分、总灰分、醇溶性浸出物进行测定;以盐酸阿替新为指标,采用紫外-可见分光光度法测定榜嘎中总生物碱的含量,检测波长408 nm。以槲皮素和山柰酚为检测指标,利用HPLC测定榜嘎中总黄酮醇苷的含量,流动相甲醇-0.1%甲酸水溶液（51∶49）,检测波长366 nm。结果：17批榜嘎药材测定结果显示榜嘎药材中水分、灰分、醇溶性浸出物、总生物碱和总黄酮醇苷质量分数分别为7.23%-10.20%,5.94%-18.60%,11.30%-29.40%,0.39%-1.99%,0.42%-3.09%。结论：建立的方法简便可行、重复性好,可用于榜嘎药材的质量控制。

Objective： In order to efficiently control quality of Ponka,its quality standard was established in this study. Method： Determination of moisture,total ash and ethanol-soluble extract in the crude drug were carried out based on methods recorded in appendix of the 2015 edition of Chinese Pharmacopoeia. The content of total alkaloids was determined by ultraviolet spectrophotometry, using atisine hydrochloride as the reference substance. The content of total flavonol glycoside was assayed by HPLC on a Welch C_（18） column,quercetin and kaempferol were used as the reference substances. Result： Contents of moisture, total ash,ethanol-soluble extract,total alkaloids and total flavonol glycoside in 17 batches samples were varied in ranges of7. 23%-10. 20%, 5. 94%-18. 60%, 11. 30%-29. 40%, 0. 39%-1. 99% and 0. 42%-3. 09%. Conclusion：These established methods are simple,feasible and reproducible,they can be used for quality control of Ponka.