不同前处理方法测定金银花中有机氯农药残留量的比较

Determination of Organochlorine Pesticide Residues in Lonicerae Japonicae Flos by Different Pretreatment

目的：通过固相微萃取（SPME）技术与其他3种前处理方法进行对比,建立一种SPME-GC快速测定金银花中有机氯农药（OCPs）残留量的方法。方法：分别采用2010年版《中国药典》方法,《日本药局方》16版方法,《2014年国家食品污染和有害因素风险监测工作手册》方法以及SPME技术测定金银花中有机氯农药残留状况,并进行回收率计算。结果：4种前处理方法的回收率均在65%-120%,其中2010年版《中国药典》方法、《日本药局方》16版方法和SPME技术杂质干扰相对较少。结论：分析了4种方法检测金银花药材中有机氯农药残留量的差异,并确定了在中药材中有机氯农药残留量测定中,SPME-GC是一种快速、简单、灵敏的检测方法。

Objective： To establish SPME-GC method for rapid determination of organochlorine pesticide residues（ OCPs） in Lonicerae Japonicae Flos by comparing solid-phase microextraction（ SPME） with other three pretreatment methods. Method： OCPs in Lonicerae Japonicae Flos were analyzed according to the methods in Chinese Pharmacopoeia 2010 edition, Japanese Pharmacopoeia 16 th revision, Manual for China National Food Contamination and Harmful Factors Risk Monitoring in 2014 and solid phase microextraction technology（ SPME）, and the recovery rates were calculated respectively. Result： Recovery rates of the four different pretreatment methods were determined between 65% to 120%. Among the methods, there was fewer impurity interference in Chinese Pharmacopoeia 2010 edition method,Japanese Pharmacopoeia 16 th revision method and SPME. Conclusion： The effect of 4 methods on OCPs in Lonicerae Japonicae Flos was analyzed,and it was determined that SPME-GC was rapid,simple and sensitive.