加味苓桂术甘汤对代谢综合征大鼠抗氧化作用及脂联素表达的影响

Effects of Modified Linggui Zhugan Tang on Oxidative Stress and Expression of Adiponectin in Rats with Metabolic Syndrome

目的:加味苓桂术甘汤对代谢综合征大鼠肾周脂肪中脂联素表达水平的影响。方法:取3周龄断乳雄性Wistar大鼠,随机分为正常组,高脂高盐模型组,阿托伐他汀降脂组(8 mg·kg^(-1)),加味苓桂术甘汤高、中、低剂量组(18,12,6 g·kg^(-1)),每组10只动物,除正常组外,其他组均给予高脂高盐饲料,并于模型制作当日分别ig给予药物治疗,正常组、模型组给予同量生理盐水,每天1次,连续喂养12周。称体重、肾周脂肪质量,计算肥胖指数及Lee's指数,生化法检测血清中超氧化物歧化酶(SOD)和丙二醛(MDA),苏木素-伊红(HE)染色观察肾周脂肪组织形态学改变,实时荧光定量聚合酶链式反应(q PCR)法检测脂肪中过氧化物酶体增殖剂激活受体γ(PPARγ)和脂联素(adiponectin)mRNA基因表达。结果:与正常组比较,模型组大鼠体重、肾周脂肪、肥胖指数及Lee's指数明显升高,脂肪细胞体积明显增加、数量明显减少,血清中MDA含量明显升高,SOD活性明显降低,脂肪组织中PPARγ和adiponectin mRNA表达明显降低(P<0.05,P<0.01);与代谢综合征模型组比较,加味苓桂术甘汤各剂量组大鼠体重、肾周脂肪、肥胖指数及Lee's指数明显降低,脂肪细胞体积明显减少、数量明显增多,血清中MDA含量明显降低,SOD活性明显增加,脂肪组织中PPARγ和adiponectin mRNA表达均明显升高(P<0.05,P<0.01)。结论:加味苓桂术甘汤具有明显改善内脏肥胖和抗氧化作用,其改善代谢综合征大鼠糖脂代谢紊乱可能与增加PPARγ促进脂联素的表达有关。

Objective： To explore the effects of modified Linggui Zhugan Tang on expression of adiponectin in perirenal fat of rats with metabolic syndrome. Method： Male weaning Wistar rats aged 3 weeks,were divided into six groups in randomized manner： normal group treated with distilled water diet; high fat and high salt model group,atorvastatin lipid-lowering group（ 8 mg·kg^-1）,modified Linggui Zhugan Tang high dose,middle dose and small dose groups（ 18,12,6 g·kg^-1）,n = 10 in each group. All other groups except normal group received high fat and high salt diet and received drugs by intragastric administration from the same time of modeling,once a day for 12 weeks. The rats in normal group and model group received the same volume of normal saline. Obesity index and Lee＇s index were calculated according to body weight and perirenal fat mass. Superoxide dismutase（ SOD） and malondialdehyde（ MDA） levels in serum were detected by biochemical method; the morphology changes of the perirenal fat tissues were observed by using HE method. The mRNA expression levels of peroxidase proliferation agent activated receptor γ（ PPARγ） and adiponectin were detected by quantitative realtime polymerase chain reaction（ qPCR）. Result： As compared with the normal group, the body weight,perirenal fat mass,obesity index and Lee＇s index in model group were significantly increased; the volume of fat cells was increased significantly while the number was significantly reduced; MDA level in serum was significantly increased; SOD activity was significantly reduced; the mRNA expression levels of PPARγ and adiponectin in fat tissues were significantly reduced（ P〈0. 05,P〈0. 01）. As compared with the metabolic syndrome model group,the body weight,perirenal fat mass,obesity index and Lee＇s index in modified Linggui Zhugan Tang all dose groups were significantly decreased; the volume of fat cells was reduced significantly while the number was significantly increased; MDA level in serum was significantly reduced; SOD activity was significantly increased;the mRNA expression levels of PPARγ and adiponectin in fat tissues were significantly increased（ P〈0. 05,P〈0. 01）. Conclusion： The modified Linggui Zhugan Tang can significantly regulate lipid oxidation and improve the visceral obesity in metabolic syndrome model rats. The mechanism may be related to increasing PPARγ expression and further increasing the expression of adiponectin.