克隆探针技术发现类风湿性关节炎新型抗原

Research on a Novel Autoantigen in Rheumatoid Arthritis

类风湿性关节炎(RA)是一种病因尚未明了的全身性自身免疫性疾病。近年来,在RA的治疗方面国际科研界有了较大的进展,但目前仍然很少有新的自身抗原被发现。本课题首次运用患者关节滑膜液中的抗体作为探针,提取RNA,进行cDNA基因库免疫筛选。结果发现新型自身抗原cDNA克隆,因其与RBP1有36.5%的氨基酸序列相同,因此命名RBP1like Protein(Rbik),此自身抗原的发现有可能为类风湿性关节炎的实验诊断和治疗提供新的可能指标和靶点。

Rheumatoid arthritis （RA） is an autoimmune disease characterized by long-term in- flammation and resultant destruction of multiple joints. In joint spaces of patients with rheumatoid arthritis （RA）, persistently presented antigens appear to play a major role in sustained in- flammation by continuously stimulating T and B cells. Synovium seems to supply most of such antigens. We cloned RBPl-like protein from synovial tissue, named Rbik,as an autoantigen in RA.Rbik has 36.5% homology with retinoblastoma binding protein 1 （RBP1） which associates with retinoblastoma （RB） tumor suppressor protein and is thought to thereby modulate the gene repression activity of RB. we report that analysis between RA and the new autoantibodies to RBPi-like Protein（Rbik）.