基于磁纳米颗粒和G-四链体DNA酶的Hg^(2+)传感器研究

Sensor for Detection of Mercury Ions Based on Magnetic Nanoparticles and G-quardruplex DNA Zymes

以磁纳米颗粒(MNPs)为固定DNA探针的固相载体,发展了一种基于分子间裂分G-四链体-血红素DNA酶以及T-Hg^(2+)-T结构的Hg^(2+)"Turn on"检测生物传感器.考察了多种因素对检测体系的影响,在最优实验条件下,该传感器体现出良好的选择性、灵敏度以及抗干扰能力,而且具有可靠的实际水体样品检测能力.以磁纳米颗粒为固相载体,可实现高效、快速及简单的分离,有效的提高检测灵敏度和降低背景信号.

Based on intermolecular biosensor for ＂Turn on＂ detection sp of lit G-quardruplex-hemin DNAzymes and T-Hg^2＋ -T binding motif, a mercury ions is developed with magnetic nanoparticles （MNPs） as carrier to immobilize the DNA probes. The effects of several factors on detection system are investigated. Under optimal conditions, this sensor displays good selectivity, sensitivity and anti-interference capability and can reliably be used for defection of real chieved by using magnetic nanoparticles as a water samples. The efficient, fast and simple separation is a solid phase carrier to effectively increase the detection sensitivity and decrease the background signal.