期刊文献+

12株禽痘疫苗毒中禽网状内皮组织增生病病毒5′LTR整合位点的比较研究 被引量:1

Comparative Analysis of Integration Sites of Reticuloendotheliosis Virus 5′LTR in 12 Fowl Pox Vaccine Strains
下载PDF
导出
摘要 为探究近年来禽痘(FP)疫苗中禽网状内皮组织增生病病毒(REV)5′长末端重复序列(LTR)的整合情况,收集2012—2014年不同地区不同批次的12株FP疫苗,稀释后接种鸡胚成纤维细胞(CEF),提取细胞DNA,扩增REV相关基因并测定序列。结果表明,12个禽FP疫苗株均扩增出含REV-5′LTR和FPV片段在内的产物,其中2株国外疫苗扩增序列长度为745bp,10株国产疫苗扩增序列长度为485bp。经DNA-Star比对分析,2株国外疫苗整合了446bp的几乎完整的REV-LTR序列,另外10株国产疫苗整合的REV-LTR片段相对较小,仅为194bp。这12个FP疫苗株中整合的REV-LTR与中国近年分离到的REV野毒株HA1101的LTR的相似性为70.3%-82.6%,与国外已发表的FP毒株AY255632整合的REV-LTR的相似性高达98.6%-100%。结果提示,国内外FP疫苗整合有REV-LTR序列的现象普遍存在。本研究检测的12个FP疫苗株均整合有REV-LTR序列,一部分疫苗株整合了几乎完整的REV-LTR序列,而大部分疫苗株整合片段则相对较小,并且与国外已分离到的FP毒株的序列同源性更接近。 To study the integration sites of avian reticuloendothliosis virus (REV)5' long terminal repeat (LTR) in fowl pox virus (FPV) vaccine strains in recent years, 12 different batches of FPV vaccines in different regions from 2012 to 2014 were collected for study. All vaccines were diluted and inoculated into chicken embryo fibroblast (CEF),DNA was extracted for the amplifi- cation and sequencing of REV relating genes. (Result) The results showed that both REV-5' LTR and FPV fragments existed in the 12 FPV vaccine strains. To be specific,745 bp in the 2 abroad vaccine strains and 485 bp in the other 10 domestic vaccine strains. By DNA-Star analysis,446 bp as almost the entire REV-LTR were integrated in the 2 abroad vaccine strains, while, a part of the REV-LTR fragment about 194 bp were integrated in the 10 domestic vaccine strains. The inte- grated REV-LTR sequence among the 12 FPV vaccine strains had 70.3%-82.6% homology with the Chinese reference strain HAll01. But it had as high as 98.6%-100% homology with REV- LTR in the published abroad PFV strain AY255632. The results suggested that the phenomenonof the integration of REV-LTR sequence m FP vaccine is, study,the 12 fowl pox vaccine strains were all integrated with REV-LTR sequence, part of the FPV vaccine strains were integrated with the complete REV-LTR sequence. While the fragment in most FPV vaccine strains was relatively small,and these sequences have a closer homology to the abroad FPV.
出处 《畜牧兽医学报》 CAS CSCD 北大核心 2016年第5期1062-1066,共5页 ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金 山东省科技发展计划(2014GNC110006)
关键词 禽痘疫苗 禽网状内皮组织增生病病毒 5′LTR fowl pox vaccine reticuloendothliosis virus 5'LTR
  • 相关文献

参考文献6

二级参考文献64

共引文献41

同被引文献5

引证文献1

二级引证文献1

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部