不同来源沙雷氏菌的分离鉴定及其耐药性分析

Isolation,identification and antibiotic resistance pattern of Serratia spp. from different sources

为说明不同来源的沙雷氏菌的分离鉴定及耐药性情况,本试验对同一地区采集的具有代表性样本即白蚁肠道、牛乳腺炎病料、污水和土壤中分离出的6株疑似沙雷氏菌进行形态学观察、生理生化试验鉴定、16S rDNA鉴定,采用微量稀释法测定6株疑似沙雷氏菌对11种抗菌药物的最小抑菌浓度(MIC),并人工感染小鼠检测菌株的致病性。结果表明,6株分离菌中分别有黏质沙雷氏菌和液化沙雷氏菌各3株。药敏试验结果表明,6株沙雷氏菌均有一定的耐药性。人工感染小鼠部分死亡,说明沙雷氏菌存在一定的致病性。

In order to explain the isolation and drug resistance situation of Serratia from dif- ferent sources,in this experiment representative samples were collected from termite gut, bovine mas- titis,sewage and soil separation,and 6 strains of suspected of Serratia marcescens by morphological observation, physiological and biochemical tests and 16S rDNA identification, ll antimicrobial mini- mum inhibitory concentration（MIC） of 6 suspected S. marcescens was determined by microdilution. The pathogenecity of the strains was determined using mice. The results showed that 6 strains of S. marcescens and S. liquefaciens were three strains. Drug sensitivity test showed that 6 strains of bac- teria Shaleishi have certain resistance. Part of the death of mice infected indicated that Serratia showed a certain pathogenicity. These will lay a certain foundation to further clarify the animal pro- duction and environmental control in the process of scientific and rational drug use, to protect the environment and prevent the importance of the spread of this resistance.