荞麦对高脂膳食大鼠肝脏中脂代谢相关酶及血清中蛋白的影响

Effects of buckwheat on lipid metabolism enzymes of liver and protein of serum in high- fat diet rat

本文研究荞麦(buckwheat,BW)对高脂膳食大鼠肝脏脂代谢相关酶及血清蛋白含量影响。将50只成年Wistar雄性大鼠随机分为基础对照组,高脂模型组,荞麦低、中、高剂量组,30 d后取血及肝脏,测定相关指标。结果表明,与基础对照组相比,高脂膳食大鼠体重增加,肝脏肝脂酶(HL)、脂肪酶(LPS)、脂蛋白脂肪酶(LPL)活性显著降低(p<0.05)。与高脂模型组相比,荞麦高剂量组大鼠体重增加量显著降低(p<0.05);荞麦中、高剂量组LPS、LPL活性显著增高(p<0.05),HL活性降低。各组大鼠血清总蛋白(TP)及白蛋白(ALB)变化差异不显著。相关性分析,荞麦粉剂量与HL、LPS、LPL活性呈现正相关关系,LPL与HL活性呈极显著正相关(p<0.01),LPS与LPL、HL活性呈显著正相关(p<0.05)。综上,荞麦干预高脂膳食可调节机体脂代谢相关酶活性。

The aim of this study was to point out the effect of buckwheat on lipid metabolism enzymes of liver and protein of serum in high- fat diet rats.Fifty male Wistar rats were assigned randomly to the following groups： control diet,high- fat diet（ HFD） and low,medium,high dosage BW group. The relevant index of livers and serum were detected after 30 days.The results showed that weight of HFD group were increased and activity of HL,LPL and LPS were decreased significantly than control diet group（ p〈 0.05）. Compared with HFD group,the increasing weight of high BW group rats were decreased（ p〈 0.05）,activity of LPL and LPS in medium and high dosage of BW group were increased significantly（ p〈 0.05）,activity of HL were decreased. TP and ALB of serum in these groups had no significant difference（ p 〈0.05）. BW and HL,LPS,LPL had positive correlation through correlation analysis,LPS had highly significant positive correlation with HL（ p〈 0.01）,LPS had significant positive correlation with LPL and HL（ p 〈0.05）.In conclusion,BW may regulate the activity of lipid metabolism enzymes by interference high fat diet.