菌剂强化对水解酸化处理模拟印染废水效果的影响

Bioaugmentation with microorganisms for hydrolysis acidification treatment of dyeing wastewater

将具有高效脱色效率的混合菌群FF(优势菌属为Proteobacteria门和Firmicutes门细菌)作为强化菌剂,通过流加菌的方式加入水解酸化反应器,对模拟印染废水进行菌剂强化。通过监测水解酸化反应器进出水的色度、COD、BOD5/COD和VFAs(挥发性脂肪酸)来反应菌剂强化效果。结果表明,经过菌剂强化,模拟印染废水的色度和COD去除率比强化前分别提高了大约15%和20%,同时,印染废水的BOD5/COD值由0.50增加到0.70,表明其可生化性得到显著提高。采用PCR-DGGE技术对菌剂强化前后水解酸化反应器中微生物多样性和群落结构进行探索。结果表明,经过菌剂强化处理,微生物多样性指数从2.17增加到2.56,表明物种丰富度增加,同时微生物群落结构发生显著变化,菌种系统发育树结果表明,菌剂强化后,不仅水解酸化系统中原来存在的优势菌种Bacteroidetes门细菌得以保持优势,同时系统中Proteobacteria门和Firmicutes门细菌得到了显著强化。

In this study,a mixed flora FF,with high efficiency of color removal,was fed into a hydrolysis acidification reactor to enhance the treatment effects of simulated dyeing wastewater. The treatment effects were reflected by decolorization,COD removal rate,BOD5/ COD value,and the production of volatile fatty acids（ VFAs）. The results show that after bioaugmentation by the hydrolysis acidification reactor,decolorization and COD-removal rate of dyeing wastewater are enhanced by approximately 15% and 20%,respectively. The value of BOD5/ COD increased from 0. 5 to 0. 7,which indicates a significant improvement in biodegradation. The diversity and structure of the microbial community in the hydrolysis acidification reactor,before and after bioaugmentation,were analyzed by PCR-DGGE. The results suggest that after bioaugmentation,Shannon-Weaver index（ H） increases from 2. 17 to 2. 56,which indicates an increase of species richness. Meanwhile,the structure of microbial community changes largely. Phylogenetic analysis indicates that Bacteroidetes sp. remain dominant,despite bioaugmentation,while the Proteobacteria and Firmicutes species are enhanced.