载吲哚菁绿和液态氟碳的光致相变型纳米粒的制备及增强体内成像的实验研究

Laser-induced phase-shift nanoparticles with ICG and PFH for photoacoustic and ultrasound imaging of tumor

目的制备一种光致相变型液态氟碳纳米粒,研究其体外相变及体内增强光声、超声成像能力。方法采用三步乳化技术制备出以聚乳酸-羟基乙酸共聚物(PLGA)为载体,液态氟碳(PFH)和吲哚菁绿(ICG)为内核的纳米粒,检测该纳米粒的粒径和电位,然后体外激光辐照激发纳米粒相变,体内观察该纳米粒增强超声及光声成像的能力。结果成功制备出包裹PFH和ICG的光致相变型纳米粒,该纳米粒平均粒径(599.2±134.3)nm,平均电位(-24.10±4.09)m V。激光辐照后,纳米粒可发生相变转变成微米级的微泡,体内增强了裸鼠移植瘤的光声及超声信号。结论制备的光致相变型纳米粒在激光作用下发生相变,并可增强体内超声、光声成像,为临床疾病的诊断提供了新的思路。

Objective To prepare a laser-induced phase-change liquid fluorocarbon nanoparticle and explore the phase-change in vitro and the ablitily to enhance photoacoustic and ultrasound imaging capability in vivo. Methods The nanoparticles with PLGA shell,PFH and ICG kernel were prepared using a three-step emulsification technique. The particle size and the potential charge of nanoparticles were detected. The capability of enhancing photoacoustic and ultrasound imaging using nanoparticles in vivo were evaluated. Results The laser-induced phase- change liquid fluorocarbon nanoparticles were successfully prepared. The average particle size was （599.2±134.3）nm and the mean surface charge was （-24.10±4.09）mV. The nanoparticles phase transformed into microbubbles when induced by laser, meanwhile,the photoacoustic and ultrasound imaging capability of tumor were enhanced. Conclusion The prepared laser-induced phase-shift nanoparticles could enhance the ability of ultrasound and photoacoustic imaging,which potentially provides a new idea in clinical diagnosis.