逍遥散对慢性束缚应激抑郁模型大鼠JAK/STAT信号通路的影响

Effects of Xiaoyaosan on JAK/STAT signal pathway in depressive rats induced by chronic immobilization stress

目的:研究逍遥散对慢性束缚应激抑郁模型大鼠杏仁核肿瘤坏死因子-α(TNF-α)及JAK/STAT信号通路的影响,以期探讨逍遥散抗抑郁的机制。方法:雄性SD大鼠随机分正常组、模型组、氟西汀组和逍遥散组,每组7只。除正常组外,其余各组每天随机时间点束缚3h,连续21d,建立慢性束缚应激抑郁模型。在造模同时,氟西汀组灌胃氟西汀水溶液0.2mg·100g-1·d-1,0.1m L/kg;逍遥散组灌服逍遥散混悬液5.98g·kg^(-1)·d^(-1),0.1m L/kg;模型组灌服等体积去离子水。造模结束后,记录旷场实验中的修饰次数、穿格数和直立次数;ELISA法检测杏仁核TNF-α、gp130、total-Jak2及total-STAT3含量。结果:1修饰次数和穿格数:模型组均少于正常组(P<0.05),而氟西汀组和逍遥散组均多于模型组(P<0.05)。2杏仁核TNF-α、gp130及total-STAT3含量:模型组均高于正常组(P<0.05),而氟西汀组和逍遥散组均低于模型组(P<0.05)。3各组大鼠杏仁核total-Jak2含量比较,差异无统计学意义。结论:逍遥散能够改善慢性束缚应激模型的抑郁样行为,其机制可能与调节杏仁核TNF-α表达及JAK/STAT信号通路有关。

Objective： To determine the effects of Xiaoyaosan on the level of TNF-α and JAK/STAT signal pathway in the amygdala of depressive rats induced by chronic immobilization stress, and study its mechanism of antidepressant effect. Methods： Male SD rats were randomly divided into 4 groups： control group, model group, fluoxetine group, and Xiaoyaosan group. Rats in all groups except for the control group suffered from a stochastic 3 hours＇ immobilization stress in a day, for 21 consecutive days. At the same time of modeling, rats in the fluoxetine group were given the fluoxetine aqueous solution by gavage at 0.2mg/100 g once a day. And, rats in the Xiaoyaosan group were given the Xiaoyaosan suspension by gavage at 5.98g/kg once a day. Additionally, rats in the model group were given the deionized water by gavage once a day. The volume of gavage was same in the 3 groups above. After modeling, all rats were carried out an open field test to observe some behavioral changes in a new environment, such as the modification time, number of crossed squares and upright time. The contents of TNF-α, gp130, totalJak2 and total-STAT3 in the rat amygdala were determined by ELISA assay. Results： 1The modification time and the number of crossed squares in the model group were all less than those in the control group（P〈0.05）. The modification time, the number of crossed squares and the upright time in both the fluoxetine group and the Xiaoyaosan group were more than those in the model group（P〈0.05）. 2The contents of TNF-α, gp130 and total-STAT3 in the model group were higher than those in the control group（P〈0.05）. The contents of TNF-α, gp130 and total-STAT3 in both the fluoxetine group and the Xiaoyaosan group were lower than those in the control group（P〈0.05）. 3There were no statistical differences in the content of total-Jak2 either between the model group and the control group or between the treatment groups and the model group. Conclusion： It comes out that Xiaoyaosan could improve the depression disorders induced by a 21 days＇ chronic immobilization stress, which might be related to regulate the expression of TNF-α and JAK/STAT signal pathway in the rat amygdala.