特应性皮炎患者外周血总IgE和T淋巴细胞亚群与中医证候的相关性探讨

Correlation of T-lymphocyte subsets, total Ig E level in serum of atopic dermatitis and its TCM syndrome types

目的:探讨青少年和成人特应性皮炎(AD)的外周血总IgE和T淋巴细胞亚群与中医证候的相关性。方法:采用流式细胞分析技术及荧光酶联免疫法对38位青少年及成人AD患者外周血T淋巴细胞亚群及总IgE水平进行测定。结果:心脾积热型患者的血清总Ig E水平最高,与心火脾虚型及脾虚湿蕴型比较差异具有统计学意义(P<0.05);脾虚湿蕴型患者的血清总IgE水平最低,与心火脾虚型比较差异具有统计学意义(P<0.05)。4个证型间患者血清中CD_3^+、CD_4^+、CD_8^+、CD_4^+/CD_8^+比较均无显著性差异。结论:青少年及成人AD的中医辨证分型与外周血总IgE的水平之间存在一定的关系,与T淋巴细胞亚群间暂未见显著性关联。

Objective： To investigate correlation of TCM syndrome types and T-lymphocyte subsets, Ig E level in serum of adolescents and adults with atopic dermatitis（AD）. Methods： The T-lymphocyte subsets and total Ig E level in serum of 38 patients with atopic dermatitis were detected by flow cytometry and fluorescent enzyme immunoassay. Results： Compared with the syndrome of heart fire and spleen deficiency and syndrome of dampness stagnancy due to spleen deficiency, the serum Ig E level in patients with syndrome of accumulated heat in both heart and spleen was significantly the highest（P〈0.05）. Compared with syndrome of heart fire and spleen deficiency and syndrome of accumulated heat in both heart and spleen, the serum Ig E level in patients with syndrome of dampness stagnancy due to spleen deficiency was significantly the lowest（P〈0.05）. There were no significantly differences in the level of CD_3~＋、CD_4~＋、CD_8~＋、CD_4~＋/CD_8~＋ ratio among the four syndrome types. Conclusion： TCM syndrome type is related to Ig E level in serum of adolescents and adults with AD nor T-lymphocyte subsets.