薏苡仁不同组分干预脾虚水湿不化大鼠细胞间隙基因变化

Data mining on genic change of intercellular space component of gene chip on rats with syndrome of fluid-dampness due to spleen deficiency intervened by different components in coix seed

目的:探讨薏苡仁不同组分对脾虚水湿不化模型大鼠空肠extracellular space组件的影响。方法:以高脂低蛋白饮食+游泳建立脾虚水湿不化大鼠模型,基因芯片技术进行空肠全基因组测定,以Quick-GO注释到的extracellular space组件进行PCA分析。结果:模型组和正常对照组具有非常清楚的差异,模型组主要出现了Sepp1、Lamc2、Csn1s1、Mmp8、Ccl20、Apoe、Apoa5、Pyy、Ccl19、Np4基因的上调和Gnrh1、Col6a3基因的下调,薏苡仁水煎液和各拆分组分均能够对模型大鼠的差异基因进行调节,改善机体微环境,主要起作用的基因是Col6a3、Sepp1、Lamc2、Gnrh1、Myoc、Csn1s1、Mmp8、Ccl20、Arg1、Apoe等。结论:脾虚水湿不化的运化失常应该是以物质由小肠上皮细胞向其他组织器官运送过程的异常为主,薏苡仁各组分的治疗都可使失衡的细胞微环境得到改善。

Objective： To evaluate the change of jejunal extracellular space components in rats with syndrome of fluiddampness due to spleen deficiency and the effect of different components in coix seed by gene chip technology. Methods： The rat model of fluid-dampness due to spleen deficiency was established by high fat and low protein diet ＋ forced swimming, the whole genome of jejunum was tested by gene chip technology, and the extracellular space components noted from Quick-GO were analyzed by PCA. Results： There had significant differences between model group and control group, the Seppl, Lamc2, Csn1s1, Mmp8, Ccl20, Apoe, Apoa5, Pyy, Ccl19, Np4 gene were up-regulated and Gnrh1, Col6a3 gene were down-regulated in model group. The decoction of coix seed and its components could regulate the extracellular space and be able to improve the body＇s micro environment. The main target gene was Col6a3, Sepp1, Lamc2, Gnrh1, Myoc, Csn1s1, Mmp8, Ccl20, Arg1, Apoe etc. Conclusion： The main disorder of transportation and transformation of spleen deficiency and fluid-dampness was the conductive abnormality from intestinal epithelial cells to other tissues and organs, the components from coix seed was able to improve the imbalance of cell micro environment.