异氟烷对间歇缺氧静脉内皮细胞的保护作用研究

Isoflurane protects human umbilical vein endothelial cells on intermittent hypoxia injury

目的探讨异氟烷对间歇缺氧(IH)人脐静脉内皮细胞的作用。方法 HUVEC和EAhy926细胞分为对照组、模型组及1%、1.5%、2%异氟烷预处理组,测定细胞活力、乳酸脱氢酶(LDH)和超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量和谷胱甘肽(GSH)水平,采用Real-time PCR和Western blot法检测低氧诱导因子(HIF-1α)和核转录因子-B(NF-κB)的表达。结果与对照组比较,模型组细胞活力、SOD活性和GSH水平降低,LDH活性、MDA含量、HIF-1α和NF-κB表达升高,差异有统计学意义(P<0.05或P<0.01)。与模型组比较,异氟烷预处理组的细胞活力、SOD活性、GSH水平和HIF-1α表达,增加LDH活性、MDA含量和NF-κB表达降低,差异有统计学意义(P<0.05或P<0.01)。结论异氟烷能清除细胞氧自由基,上调HIF-1α表达,下调NF-κB表达,提示其能降低IH引起氧化应激和炎性反应,减轻内皮细胞IH损伤。

Objective To study the effect of isoflurane on intermittent hypoxia （IH） injured human umbilical vein endothelial cells. Methods HUVEC and EAhy926 cells were divided into control group, model group, 1%, 1.5%, 2% Isoflurane pre-treatment group. Cell viability, MDA and GSH levels, LDH and SOD activities, and HIF-lot and NF-KB expressions were tested by MTY assay, expression of MDA, LDH, SOD and GSH were tested by Real-time PCR and Western blot, respectively. Results Compared with control group, the cell viability, SOD activity and GSH level de- creased, LDH activity and MDA level, and expression of HIF-Iot and NF-KB increased in model group cells, the dif- ferences were statistically significant （P 〈 0.05 or P 〈 0.01）. Compared with model group, the cell viability, SOD activ- ity, GSH level and HIF-let expression increased, MDA level, LDH activity and NF-KB expression decreased, the dif- ferences were statistically significant （P 〈 0.05 or P 〈 0.01） Conclusion Isoflurane protects human umbilical vein en- dothelial cells from IH injury by scavenging free radical and reducing oxidative stress and inflammation.