子痫前期患者血浆中Ghrelin与VEGF、sFlt-1的相关性

Correlation of serum Ghrelin with VEGF and s Flt-1 in patients with preeclampsia

目的研究子痫前期患者血浆中Ghrelin水平与血管内皮生长因子（VEGF）和可溶性Fms样酪氨酸激酶-1（s Flt-1）表达的相关性。方法 选取2013年12月~2015年6月于复旦大学附属上海市第五人民医院产科住院分娩的子痫前期患者36例、正常妊娠妇女36例为研究对象,分别设为子痫前期组和对照组。采用酶联免疫吸附试验检测两组妇女血浆Ghrelin、VEGF、s Flt-1浓度,并分析其相关性。结果 与对照组比较,子痫前期组患者血浆中s Flt-1水平明显升高（P〈0.05）,而VEGF水平明显降低（P〈0.05）。与对照组比较,子痫前期组患者血浆中Ghrelin水平明显升高（P〈0.05）,Ghrelin与VEGF呈正相关（r=0.837,P〈0.05）,与s Flt-1呈负相关（r=-0.797,P〈0.05）。结论 子痫前期患者血浆中Ghrelin水平与VEGF、s Flt-1具有相关性,提示Ghrelin可能通过影响内皮细胞功能参与子痫前期的发生、发展。

Objective To investigate the association of Ghrelin with vascular endothelial growth factor （VEGF） and serum soluble Fms-like tyrosine kinase-1 （sFh-1） in patients with preeclampsia. Methods 36 cases of preeclampsia and 36 cases of normotensive pregnancy who given birth in the Department of Obstetrics of the Fifth People＇s Hospital of Shanghai, Fudan University from December 2013 to June 2015 were selected, and they were divided into preeclamp- sia group and the control group. Levels of Ghrelin, VEGF and sFh-1 proteins in plasma of two groups were detected by enzyme linked immunosorbent assay, the relationships between them were analyzed. Results Levels of sFlt-1 in preeclampsia group were significantly higher （P 〈 0.05）, whereas the levels of VEGF in preeclampsia group were significantly lower than those in control group （P 〈 0.05）. Compared with the control group, levels of Ghrelin in preeclampsia group was significantly higher （P 〈 0.05）. There was obviously positive correlation between levels of Ghrelin and VEGF in preeclampsia group （r = 0.837, P 〈 0.05）, and negative correlation between levels of Ghrelin and sFlt-1 （r = -0.797, P 〈 0.05）. Conclusion Ghrelin is closely correlated with levels of VEGF and sFlt-1 in preeclampsia serum. Ghrelin may participate in the occurrence and development of preeclampsia by influencing the endothelial cell function.