源于大麦小孢子突变体的苗期耐盐性研究

Study on the salt tolerance of mutants derived barley microspores at seedling stage

以诱变结合小孢子培养获得的6份大麦突变体及其野生型亲本'花30'为供试材料,采用营养液盐胁迫水培方法,测定了苗期的形态指标(株高、分蘖数、干重)和不同部位(根、茎叶)钠/钾离子的含量,并对其耐盐性进行了评价。结果表明:盐胁迫下6份大麦突变体及其亲本苗期生长受到严重影响,株高、分蘖数和干重等形态指标均呈不同程度下降;不同突变体间以及突变体与亲本间在多个测定指标上存在差异,形态指标耐盐性综合评定3份突变体(A7-6、A7-12和A7-20)耐盐性优于亲本'花30',地上部K^+/Na^+含量比例表明2个材料(A7-6和A7-20)耐盐性优于亲本'花30',综合比较认为1份突变体(A7-20)苗期耐盐性优于亲本'花30'。

The six barley mutants obtained from the mutation combined with microspore culture, as well as their wild type variety＇ Hua-30＇ were used as materials in this study. Under the salt stress by hydroponic culture method, the morphological indexes （shoot height, tiller numbers, dry weight）and the contents of sodium and po- tassium （ roots and shoots） at seedling stage were determined to evaluate the salt tolerance of the test materials. The results showed that the 6 barley mutants and their parent were severely affected by salt stress, and the shoot height, tiller numbers and dry weight showed varying degrees of decline. There were differences of morphological indexes among the different mutants and among the mutants and the parent. The salt tolerance of 3 mutants （A7- 6,A7-12 and A7-20）was better than that of the parent ＇ Hua-30＇ by comprehensive evaluation of salt tolerance of morphological indexes. The ratio of K ＋/Na ＋ content above the ground showed that the salt tolerance of 2 materials （A7-6 and A7-20） was better than that of the parent ＇ Hua-30＇. With comprehensive comparison, the salt tolerance of the mutant A7-20 was superior to the parent＇ Hua-30＇ at seedling stage.