不同培养方法对松材线虫鉴定的影响

Effect of different culture method on the identification of Bursaphlenchus xylophilus

开展了松萎蔫病疫木(枯死木、包装板)不同温度、时间保湿培养,以真菌为培养基培养松材线虫分散型3龄幼虫对松材线虫鉴定率影响的研究。结果表明:随着培养温度提高、时间的延长,松材线虫鉴定率随之升高。疫木保湿培养,48 h之前,鉴定率呈缓慢上升趋势,48 h之后鉴定率快速上升;疫木保湿30℃培养72 h或25℃培养96 h,真菌30℃培养72 h或20-25℃培养96 h,松材线虫分散型3龄幼虫样本的松材线虫鉴定率达100%,2种培养方法均适宜在松萎蔫病疫木松材线虫鉴定中应用。

The effect of culturing dispersion-type three-instar-larvae of Bursaphlen~＇hus xylophilus was studied on the identifi- cation rate, i.e. moisturizing culture of wood （dead standing tree or packing board） infected by pine wilt disease and on the medium containing fungi under different temperature and time duration. The results showed the identification rate of B. xylo- philus increased with the temperature and time duration. As for the moisturizing culture of the infected wood, the identifica- tion rate increased gently before 48 hours and rapidly after 48 hours. For the moisturizing culture of the infected wood at 30 ℃ lasting 72 hours or 25 ℃ lasting 96 hours, or the fungus medium culture at 30℃asting 72 hours or 20-25 ℃ last- ing 96 hours, the identification rate for the dispersion-type three-instar-larvae of B. xylophilus reached 100%. Such two methods are suitable for the identification of B. xylophilus from the wood infected by pine wilt disease.