5，7-二甲氧基黄酮抑制人胰腺癌PANC-1细胞系胰腺癌干细胞特性的体外研究

5,7Dimethoxyflavone Inhibits the Properties of Human Pancreatic Cancer Stem Cells in Pancreatic Cancer Line PANC1 in Vitro

【目的】探讨5,7二甲氧基黄酮（DMF）对人胰腺癌干细胞特性的影响。【方法】体外培养人胰腺癌PANC1细胞系得到微球细胞，流式细胞仪检测；划痕法分析不同浓度DMF对胰腺癌干细胞自我更新、体外细胞迁移和运动能力影响； Western Blot分析不同浓度DMF干预后PANC1细胞系胰腺癌干细胞表面标志物CD133、CD44、ALDH1、Bmil、Ecad、Ncad蛋白表达变化。【结果】DMF以浓度依赖方式抑制人胰腺癌PANC1细胞系胰腺癌干细胞肿瘤球形成能力及抑制细胞体外迁移能力；DMF以浓度依赖方式使胰腺癌干细胞标志物CD133、CD44、ALDH1、Bmi1、Ncad蛋白表达水平降低,而Ecad蛋白水平升高。【结论】DMF以浓度依赖方式显著抑制人胰腺癌PANC1细胞系胰腺癌样干细胞自我更新、分化后细胞迁移、细胞标志物CD133、CD44、ALDH1、Bmi1、Ncadherin蛋白表达，而增强Ecadherin蛋白表达。

【Objective】To examine the effects of 5,7Dimethoxyflavone （DMF） on the properties of human pancreatic cancer stem cell. 【Methods】Human pancreatic cancer PANC1 cell lines were cultured in vitro to generate mammospheres and then detect phenotypes by flow cytometry. The Scratch method was used to contrast cell migration and movement ability after the influence of different concentrations of DMF on the pancreatic cancer stem cells in vitro. Western blot analysis was used to compare of surface marker CD133, CD44, ALDH1, Bmil, Ecad, and Ncad protein expression levels of human PANC1 pancreatic cancer stem cells after different concentrations of DMF interaction.【Results】DMF in a concentration dependent scenario decreased the sphereforming and migration ability of human PANC1 pancreatic cancer stem cells in vitro. Stem cell marker CD133, CD44, ALDH1, Bmi1, and Ncad protein expression levels were decreased by DMF in a concentration dependent manner, but Ecad protein level increased. 【Conclusion】DMF in a concentration dependent manner significantly inhibited in human PANC1 pancreatic cancer stem cell selfrenewal, cell migration, stem cell markers CD133、 CD44、 ALDH1、 Bmi1 protein expression ,but Ncadherin protein expression increased.