鸟分枝杆菌脂类诱导的人肺泡巨噬细胞抗结核免疫反应性的改变及机制的初步研究

Mycobacterium avium-derived lipids attenuate immune response against Mycobacterium in human alveolar macrophages

目的研究鸟分枝杆菌脂类(MALs)对人肺泡巨噬细胞抗结核免疫反应性的影响。方法平板计数法评价MALs对人肺泡巨噬细胞杀结核菌效应的影响;ELISA检测肿瘤坏死因子-α(TNF-α)水平;Greisse法检测一氧化氮(NO)水平。结果MALs减弱了人肺泡巨噬细胞对牛结核分枝杆菌(BCG)的杀灭。MALs刺激后,结核分枝杆菌纯蛋白衍生物(PPD)和干扰素γ(IFN-γ)诱导的人肺泡巨噬细胞TNF-α和NO的分泌均有所下降。结论 MALs减弱了人肺泡巨噬细胞对结核分枝杆菌的免疫反应性。

Objective To evaluate the effects of exposure to MALs on immunity against Mycobacterium tuberculosis in human alveolar macrophages.Methods Plate counting were used to evaluate the effect of resisting Mycobacterium tuberculosis in human alveolar macrophages.Culture supernatants were harvested and human TNF-αwas measured by ELISA kit.Total nitrite levels in the media were measured using the Griess reagent.Results MALs stimulation attenuated the control of intracellular mycobacterial growth in human alveolar macrophages.The production of proinflammatory cytokines TNF-αand Nitric oxide in human alveolar macrophages induced by Mycobacterium tuberculosis purified protein derivatives tuberculin（Mt-PPD）and human interferonγwere attenuated by pretreatment in vitro with MALs.Conclusion MALs attenuated the responses of human alveolar macrophages against Mycobacterium tuberculosis,which may provide the new evidence to explain the phenomena in which some component of Mycobacteria in environment impacts the immunity against Mycobacterium tuberculosis infection.