摘要
目的探讨水通道蛋白3对胃癌细胞株SGC7901中基质金属蛋白酶的调节作用。方法构建水通道蛋白3的过表达及干扰质粒，由慢病毒作载体分别转染SGC7901细胞，随后检测SGC7901细胞中MMP表达的变化。结果SGC7901细胞的AQP3被敲除后，MTl-MMP、MMP-2、MMP-9的表达显著降低，而当其中AQP3过表达时，MTl-MMP、MMP-2、MMP-9的表达又明显增加；SGC7901细胞中AQP3的沉默可导致AKT途径ser473磷酸化水平降低。结论人胃癌细胞SGC7901中水通道蛋白3经P13K／AKT途径正向调节基质金属蛋白酶的表达。
Objectives To investigate the role of water channel protein 3 in the regulation of matrix metal- loproteinases in human gastric cancer cell line SGC7901. Methods In this study, AQP3 was silenced or over- expressed in SGC7901 cells. Results We found a significant decrease in MT1-MMP, MMP-2, and MMP-9 ex- pression after AQP3 knockdown, and a significant increase in MT1-MMP, MMP-2, and MMP-9 expression after AQP3 over-expression in SGC7901 cells. We also found that AQP3 silence led to a significant decrease of phos- phorylation of set473 in AKT in SGC7901 cells. Conclusion Our findings showed that AQP3 might positively reg- ulate MMPs proteins expression through PI3IGAKT signal pathway in human gastric carcinoma SGC7901 cells.
出处
《常州实用医学》
2016年第2期71-74,共4页
CHANGZHOU PRACTICAL MEDICINE
