工布乌头提取物提高果蝇肠道免疫功能的实验研究

Improvement effect of extracts from Aconitum kongboense on intestinal immune function of Drosophila melanogaster

目的研究工布乌头提取物对果蝇肠道免疫功能的影响。方法用标准的玉米粉酵母培养基饲养的果蝇作为对照组,标准培养基加入1.0%工布乌头提取物饲养的果蝇作为实验组,利用十二烷基硫酸钠(SDS)、葡聚糖硫酸钠(DSS)、过氧化氢(H2O2)和百草枯(PQ)等诱导肠道损伤,通过比较果蝇生存率、肠道上皮细胞凋亡情况、活性氧(ROS)量、肠道干细胞数量及肠道形态变化等,探讨工布乌头提取物对果蝇肠道的保护作用。结果 1.0%工布乌头提取物可以提高SDS、DSS、H2O2和PQ等致炎因子诱导的果蝇生存率;降低由SDS诱导的果蝇肠道细胞凋亡数量和肠道细胞内ROS量,减少肠道上皮细胞过度增殖,减少黑色素瘤的产生,并改善肠道形态。结论工布乌头提取物对致炎因子诱导的肠道损伤具有较好的保护作用。

Objective To study the effects of extracts from Aconitum kongboense on gut immunity of Drosophila melanogaster. Methods Flies fed with standard cornmeal-yeast medium were used as control, and the treatment groups contained 1% of A. kongboense aqueous extracts in standard medium. D. melanogaster was treated by some toxic compounds such as sodium dodecyl sulfate （SDS）, DSS, H202, and Paraquat （PQ） with or without extracts ofA. kongboense, and the effect ofA. kongboense on the survival rate, intestinal epithelium cell death, relative contents of reactive oxygen species （ROS） in intestinal epithelium cells, and the number of intestinal epithelium cells and intestinal morphology changes were analyzed. Results A. kongboense could significantly improve the survival rate of SDS, DSS, H2O2, and PQ-infected D. melanogaster, reduce the intestinal epithelial cell death and the level of intestinal ROS, decrease the over-proliferation of intestinal epithelial cell and the formation ofmelanotic masses. Conclusion The extracts of A. kongboense could significantly improve the injury induced by chemical substances. This result provides experimental foundation for the exploitation ofA. kongboense.