4种温里药对兔回肠平滑肌张力及Ca^(2+)-ATP酶的影响

Effects of Four Interior-warming Drugs on the Tension of Ileum Smooth Muscle and Ca^(2+)-ATPase in Rabbits

目的探讨高良姜、肉桂、吴茱萸、干姜4种温里药对家兔体外回肠平滑肌张力及细胞膜上Ca^(2+)-ATPase活性的影响。方法利用BL-420E+生物信号采集处理系统,记录高良姜、肉桂、吴茱萸、干姜水煎液对正常肠肌和氯化乙酰胆碱(ACh)、氯化钡(BaCl_2)、磷酸组胺(His)所致体外肠肌收缩及对ACh诱导兔回肠平滑肌内钙释放和外钙收缩的影响,测量给药前1 min内和给药后3 min内的平均张力,并根据平均张力值计算抑制率。用定磷法测定高良姜、肉桂、吴茱萸、干姜含药血清对肠平滑肌细胞膜上Ca^(2+)-ATPase活性的影响。结果高良姜、肉桂、吴茱萸、干姜高浓度组均能显著抑制正常情况下体外肠收缩(P<0.05或P<0.01);各味药低、中、高浓度组对ACh、His及BaCl_2所引起的体外肠收缩均有显著抑制作用(均P<0.01),抑制率与药物终浓度呈一定量效关系;对ACh诱导的依内钙性收缩和依外钙性收缩均有显著抑制作用(P<0.05或P<0.01);正常对照组和高良姜、肉桂、吴茱萸、干姜高浓度组肠平滑肌Ca2+-ATPase活性分别为(0.384±0.070),(0.302±0.016),(0.307±0.016),(0.296±0.016),(0.313±0.003)U·mg-1(均P<0.01)。结论温里药可能通过减少细胞内钙释放,减少细胞外钙经由受体操纵性钙通道内流,同时抑制平滑肌Ca2+-ATPase活性,从而降低细胞内[Ca2+],舒张肠平滑肌。

Objective To investigate the effects of four interior-warming drugs（ galangal,cinnamon,evodia rutaecarpa,and dried ginger）on the tension of ileum smooth muscle and Ca^2＋-ATPase on the cell membrane in rabbits. Methods The effects of galangal,cinnamon,evodia rutaecarpa,and dried ginger were examined on normal ileum smooth muscle,in vitro intestinal muscle contraction caused by acetylcholine（ACh）,barium chloride（BaCl2 ）and histamine（His）, and ACh-induced calcium release by using BL-420E＋ biological signal collection and processing system.The average tension was measured within 1 min before delivery and within 3 minutes after the treatment,and the inhibition rate was calculated according to the average tension value.The effects of sera containing galangal,cinnamon,evodia rutaecarpa,and dried ginger on Ca^2＋-ATPase activity on the cell membrane of the intestinal smooth muscle were examined by phosphorus method. Results Galangal,cinnamon,evodia rutaecarpa,and dried ginger at high concentrations could restrain in vitro intestinal contraction in normal circumstances（P〈0.05 or P〈0.01）.Significant inhibitory effects on intestinal contraction caused by ACh,His and BaCl2 were found in low,medium and high concentration groups（P〈0.01）.There was a dose-effectiveness relationship between the inhibition rate and final drug concentrations.The ACh-induced intracellular and extracellular calcium dependent contraction were significantly inhibited by the four interior-warming drugs（ P 〈 0. 05 or P 〈 0. 01）. The Ca^2＋-ATPase activities were（ 0. 384 ± 0.070）,（0.302±0.016）,（0.307±0.016）,（0.296±0.016）,（0.313±0.003）U＆#183;mg^-1 ,respectively,in intestinal smooth muscle in normal control group and high concentration groups of galangal,cinnamon,evodia rutaecarpa,and dried ginger（P〈0.01）. Conclusion Interior-warming drugs may relax intestinal smooth muscle by reducing the intracellular calcium release and the extracellular calcium inflow via receptor-controlled calcium channels,and inhibiting the Ca^2＋-ATPase activity in smooth muscle.