田间接种固氮类芽孢杆菌对小麦的增产效应

Effect of nitrogen-fixing Paenibacillus spp.on wheat yield

为了解固氮类芽孢杆菌对小麦的增产效果,对实验室分离保存的4株固氮类芽孢杆菌菌株1-18、1-33、1-43及1-49,在固氮酶活性、吲哚乙酸（IAA）分泌量及接种小麦后的增产效果进行了比较。结果表明,这4株固氮类芽孢杆菌接种小麦后,比不接种固氮菌剂的对照增产5.1%~26.9%,其中菌株1-18的增产效率最高（增产26.9%）,其次是菌株1-49（增产18.7%）。相应地,这2株菌株的固氮酶活性也比其他2株菌株高,菌株1-18固氮酶活性为1 043.6nmol/（mg·h）,菌株1-49为969.5nmol/（mg·h）,但分泌的IAA量低于其他2个菌株,菌株1-18分泌IAA量为24.95μg/mL,菌株1-49分泌IAA量为26.34μg/mL。结果说明用于本研究的固氮芽孢杆菌在固氮酶活性、IAA分泌量及对小麦的增产效果方面存在差异。

Four nitrogen-fixing Paenibacillus strains 1-18,1-33,1-43 and 1-49 are used to inoculate wheat,and the wheat yields are analyzed to determine the effect of these strains on wheat yields.The results showed that wheat yields were increased by 26.9%,9.7%,5.1% and 18.7%,respectively.Furthermore,the nitrogenase activities and IAA amounts produced by these 4strains were analyzed.Of these 4strains,strain 1-18 exhibited the highest nitrogenase activity（1 043.6nmol/（mg·h））and the activity（969.5nmol/（mg·h））of strain 1-49 is higher than the rest 2strains1-33（（565.9±3.0）nmol/（mg·h））and 1-43（（904.5±4.3）nmol/（mg·h））.However,the IAA amounts produced by strains 1-18（（24.95±1.2）μg/mL）and 1-49（（26.34±4.2）μg/mL）are lower than those produced by strains1-33（（27.73 ±1.8）μg/mL）and 1-43（（49.91±3.6）μg/mL）.Our study provided fundamental information for strain improvement of nitrogen-fixing strain for microbial fertilizer.